Basic information |
Official Symbol | WNK1 |
Description | WNK lysine deficient protein kinase 1 |
Other Aliases | HSAN2, HSN2, KDP, PRKWNK1, PSK, p65 |
Other Description | WNK lysine deficient protein kinase 1 isoform; erythrocyte 65 kDa protein; prostate-derived sterile 20-like kinase; protein kinase with no lysine 1; serine/threonine-protein kinase WNK1 |
Mutation type | Insert and frame-shift 243-244:T-T^C, Insert and frame-shift 724-725:V-P^C, Insert and frame-shift 740-741:L-F^C, Insert and frame-shift 759-760:C-P^C |
Location | Scaffold141530_1:889407 1066523 + |
Wiki | Wikipedia GeneCard |
Sequence |
WNK1 |
GGGGGAGGAGGGGACCCCCCGCCGGCCAGAAGCGGCAGCAGCGGCGGCGC CAAGGAGCCCCAGGAGGAGCGGAGCCAGCAGCAGGATGATAACGAGGAGC TGGAGACCAAGGCCGTGGGCATGTCCAACGACGGCCGCTTTCTCAAGTTC GACATCGAGATCGGCAGGGGCTCCTTTAAGACGGGCTACAAAGGCCTGGA CACCGAAACCGTGGAAGTGGCCTGGTGCGAGCTGCAGGATCGAAAGTTAA CAAAATCTGAGAGGCAGAGATTTAAAGAAGAGGCTGAAATGCTAAAGGGT CTTCAGCATCCCAATATTGTTCGATTCTACGATTCCTGGGAATCCACAGT AAAAGGAAAGAAGTGCATTGTTTTGGTGACTGAACTTATGACATCCGGAA CACTTAAAACGTACCTGAAAAGGTTTAAAGTGATGAAGATCAAAGTTCTA AGAAGCTGGTGCCGTCAGATTCTTAAAGGACTTCAGTTTCTTCATACTCG AACTCCACCTATTATTCACCGGGATCTTAAATGTGACAACATCTTTATCA CTGGCCCTACAGGCTCAGTCAAGATTGGAGACCTTGGTCTGGCGACTCTG AAGCGGGCGTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTCATGGC ACCTGAGATGTATGAAGAGAAATATGATGAATCTGTGGATGTCTATGCTT TTGGGATGTGCATGCTTGAGATGGCTACATCTGAATATCCATACTCAGAG TGCCAAAATGCTGCGCAGATCTACCGTCGAGTGACGAGCGGAGTAAAGCC AGCCAGTTTTGACAAAGTTGCCATTCCTGAAGTCAAAGAAATTATTGAAG GATGCATACGACAAAACAAAGATGAAAGATACTCCATCAAAGACCTTTTG AACCATGCCTTCTTTCAGGAGGAAACAGGGGTACGGGTAGAATTAGCAGA AGAAGATGATGGAGAAAAGATAGCTATTAAATTATGGCTACGTATTGAAG ACATTAAGAAACTAAAGGGAAAATACAAAGACAATGAAGCTATTGAGTTT TCCTTTGACTTGGAGAGAGATGTGCCAGAAGATGTTGCTCAAGAAATGGT TGAGTCTGGGTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTA TCAAAGATCGAGTGTCATTAATCAAGAGAAAACGGGAGCAGCGACAGTTG GTACGCGAGGAGCAAGAAAAAAGAAAGCAGGAAGAGAGCGGTCTCAAACA GCAGGTGGAACAGCAATCAAGTGCTTCCCAGACCGGAATCAAGCAGCTCC CGTCTGCTGGCACCGGCATCCCCACTGCTTCCACCACTTCAGCTTCAGTC TCTACACAGGTAGAACCTGAAGAACCTGAGGCAGATCAGCACCAACAACT GCAGTACCAGCAGCCTGGCGCATCTGTGTTATCTGATGGGACAGTTGACA GTGGTCAGGGATCTTCTGTCTTCACCGAATCTCGAGTTAGCAGCCAGCAG ACAGTTTCATATGGTTCCCAACATGAACCGACACATTCTACTGGCTCAAT GCCAGGGCACACAGCTTCTGTCGTCCAAGCACAGTCTCAACTCCATGGGG TATATCCACCCTCAAGTGTGCCTCGTCGTGGCCGTAGCATGTCGGTTTGT GTTCCCATGTTTCTGCTGCTCCCTCTCTGTCCCGCATCTTTCCCAGTGCT CTTCCACCCCACCACCAGTGCTGTCTGCACCATTTTCTCCTTCCCTCCTT CGGACTGCCCTGAGGAAACTTTTGCCGAAAAGCTCTCTAAAGCACTGGAG AGTGTCCTGCCTATGCACTCTGCCTCCCAGCGCAAGCACCGACGCTCCAG CCTGCCTTCCCTCTTTGTCAGTACTCCTCAGTCCATGGCGCATCCGTGTG GGGGGACCCCAACCTACCCAGAATCACAGATATTTTTCCCAACTATTCAT GAACGTCCAGTTTCTTTTTCACCACCTCCTACCTGTCCACCGAAAATAGC CATTTCTCAGCGGCGTAAGAGCACCTCCTTCCTGGAAGCCCAAACTCGCC ACTTCCAACCCCAGCTAAGGACTGTTGGCCAAAATCTTCTTCCACCTGGT GGCAGCCCATCTAACTGGACACCAGAGGCCGTAGTTATGTTGGGTCCTTC AGCCAGTAGAGTAACTGGAGAGCCATGTGAGATACAGGTCCATCCTATGT TTGAACCAACTCAAGTCTACAGTGACTATAGACCTGGACTAGTACTTCCA GAAGAAGCTCATTATCTTATTCCTCAGGATGCAGTGTTTCTAGCTGGGGT GCATTACCAGGCCCAGGTGGCAGAACAGTATGAGGGCATCCCATACAACT CACCAGTACTGTCAAGTCCTATGAAACAGATACCTGAACAGAAGCCAGTA CAAGGGGGTCCTACCTCAAGTTCTGTCTTTGAATTTCCATCTGGACAGGC TTTCCTGGTAGGACACCTTCAGAATTTAAGATTAGATTCTGGATTGGGTC CAGGATCTCCCCTTTCTAGTATTTCTGCACCTCTCAGTACAGATGCTACA TGTTTGAAATTTCATCCTGTCTTTGTTCCTCATTCTGCACCCGCTGTGTT AACTCATAGCAATGAGAGCAGAAGCAACTGTGTATTTGAATTTCATGTTC AAACACCAAGCTCCTCTTCAGGAGAAGGAGGTGGAATTTTACCTCAGCGT GTGTACCGAAATCGACAGGTTGCGGTGGACTTAAACCAGGAAGAACTGCC TCCTCAATCGGTTGGATTACATGGCCACCTGCAGCCTGTGACTGAAGAAC AGCATAATTACCAGGCCCCAGAATTGACCATTTCTGTGGTAGAGCCTGTC GGACAGAACTGGCCAATAGGAAGCCCAGAATATTCCAGTGATTCCTCACA AATCACTTCTTCAGACCCCAGTGACTTTCAGTCACCTCCCCCTACAGGGG GAGCAGCAGCACCTCTTGGCTCTGAAGTCTCATTACCCTTTATCCGACTG CCTCAGACAGTGTTACAAGAATCCCCACTCTTCTTCTGTTTTCCCCAAGG AACCACATCTCAGCAGGTCTTATCTGCCTCATTTTCTCCAGGAGGATCTG CACTCCATCCACAGGCGCAGGGGCAGAGCCAGGGGCAGCCACCCTCAAGC AGTTTAACAGGGGTTCCATCTTCCCAACCCATACAGCACCCTCAGCAGCA GCAGGGAATACAGCAGACAGCCCCTCCGCAACAGACAGTGCAGTATTCAC CTCCCCAGACGTCAGCCTCCAATGAGGCCCCTGCTGCACAGCCAGTGAGT CAGCCTCAGACGACACAGGTCTTGCCTCCAGTATCAGCTGGAAAACAGCT TCCAGTTTCCCAGCCAATACCAACTATTCAAGGCGAACCTCAGATACCAG TCGCGACACAACCCTCAGTTGTTCCGGTTCACTCTGGTGCTCATTTCCTC CCTGTGGGACAGCCACTTGCTCCTTCCTCACTCCCTCAGTACCCTCTCTC TCAAACTCCCATATCGGCTCCTCCCATGCCTACTGTGCAGACAGGTTTCT CATCCCTTCCTGTCACAATGGCAGCTGGCATTAACCAGCCTCTGCTCACC CTGGCTTCATCTGCTCCAGCAGCTGCGGTCCCAGGGGGATCGGCTGTGGT TGCTAGTCAGCTGCCAACCCTTCTTCAGCCCGTGGCTCAGCTGCCAAGGC AGACTCACCCACAGCTCCTACAGCCAGCAGTTCAGTCCATGGGAATACCA GCTAACCTTGGGCAAGCTGCTGAGGTGCCACTTCCCTCTGGAGATGTTCC GTTTCAGGGCTTCCCACCTCGACTGCCACCACAATACCCAGGAGATTCAA ACATTGTTCCCTCTTCCAGCGTGGCTTCTGTTTGCATCCATTCTACAGTC CTTTCCCCTCCCATGCCAACAGAAGCATTGGCTACACCAAATTACTTTCC TACTATGGTGCAGCCTTATGTGGAATCAAATCTTGTGGTTCCTGTGGGCG GTGTAGGAGGACAGGTTCAAGTGTCTCAGCCAGCAGTGAGTTTAGCACAG CAAGCCCCCACTGCATCCTCCCAGCCAGCAATTCTGGAGAGTACTCAGGG AGTCTCTCAGGTGGCTCCTACAGAGCCAGTTCCGGTAGCACAGCCTCAGC CTCCCCAGCCTGCTACTTTGGTGTCCTCCATAGATAGTGCACATTCCGAT GTCGCCTCTGGTATGAGTGATGGCAATGAGAATGTCCCATCATCTGGTGG GAGACATGAAGGAAGAGCTCCAAAGCGGCACTATCGAAAATCTGTACGGA GTCGCTCCCGGCATGAGAAGACGGCACGCCCCAAACTGAGAATTTTGAAT GTTTCAAATAAAGGAGACCGGGTAGTGGAATGTCAGTTAGAAACTCATAA TCGGAAAATGGTCACGTTCAAATTTGACCTAGATGGTGATAACCCTGAGG AGATAGCAACGATTATGGTGAACAATGACTTTATTCTAGCAATAGAGAGA GAGTCGTTTGTGGATCAAGTACGGGAAATCATTGAAAAAGCTGATGAAAT GCTCAGTGAGGATGTCAGTGTGGAACCCGAGGGTGACCAGGGATTGGAGA ATCTACAGGGAAAGGATGACTATGGCTTTTCAGGCTCTCAAAAATTGGAA GGAGAGTTTAAACAGCCAATGCCTGCGTCTTCCATGCCACAGCAAATAGG CGTTTCTACCAGTTCTTTAACTCAAGTTGTTCATTCTGCTGGAAGACGGT TTATAGTGAGTCCTGTGCCAGAAAGTCGATTACGAGAATCGAAACTTTTC ACCAGTGAAATACCAGATCTGGTTGCTGCCTGTACATCTCAAGGCCCTGG GATGAACTTGTCTCACTCTGCTTCATCCCTTAGTCTACAACAGGCCTTTT CCGAACTTAGACATGCCCAAATGACAGAAGGACCCAGTACAGCACCTCCG AACTTCAGTCAGACGGGGCCAGCGTTTCCCGTTGTACCTCCCTTCTTGAG TAGTGTTGCTGGGGCCCCAACCACAGCATCCGCCATACCTTCAGTATCTG TCAGTGCCACAGGCAGTCTTCTGGGTGACGTTGCCACATCAGCAACTCAT CCTGAGGTGACAGTACCCGCTGAGGCGGGGATGGCTGGAGTTGTCACTAG CACAGGTGTGATAACTCCAAGTGGTCTCCCTGTGCCACTTGTGTCTGAGT CGCCAGCACCCTCCAGTGTAGTCTCAGGCACCACTGTACCTGCCGTGGTC CCGGTACCAACATCGCAGTACACTCAGGCCCCCACGTCTTACACTCAGGC CCCCACGTCTGGAAGCACTGTTTCCAGTACTGGCACCGGTCCTTCCATAA CAACCCCTGCAGTGGGCAGCGCTGCTGCCCCCGACGCTAAGCCTCCAGCT GTGTTACCTCAGCAGACAGCAGGCAGTAGTAGTGCTGGGACAGCCGCCTT AACAGCGGTTCCTACCACCATTCCCTTCTCCAGTGTAGCTGCCCACCCGT CCCTGCAGCTTAGCAGCAGCACCTCAGCTCCTACTCTAGCTGAAACAGTG GTGGTCAGTGCACACTCTCTAGATAAAGTGTCTCATAGCAGTACAGCTGG GCTGGCGCTGTCCCTCTCTGCTCCATCATCCTCTTCCCCTGGAATAGGGC TGTCTAGTTCTGTTTCTCAGCCTGCTGGGGTACATCCTTTGGTGGTGCCA TCAGCTATATCCTCTGCTCCTGTTCCCCATGCAGCAGGACTGACATCTAC ACCTTTACTGCCCCAGGTACCCAGTGTCCCCCCCTTGGTACAGCCTGTTG CCAATGTGCCTGCTGTGCAGCAGACTCTGGTTCATAGTCAGCCACAGCCA GCTTTGCTTCCCAGCCAGCCCCATACTCACTGTCCTGAAATTGACACCGA TACTCAACCCAAAGCTCCTGGGATTGATGACATAAAGACCCTGGAGGAAA AACTGCGATCTCTCTTCAGTGAACACAGCTCATCAGGAGCACAGCATGCC CCTGTCTCACTGGAGACGTCGTTGGTAGTAGAGACAGCTGTCACACCAGG CGTCCCAACCACTGCTGTTGCACCAAGCAAGCTCATGACTTCCACTACAA GCACTTGCCTACCGCCTGCAAGCTTGTCCTTGGGAACAGCTGGTTTGCCG GTTATACCAGTGGGCACACCTGGGCAAACTTCTGCTCCAGTGAGCACCGC ATCAGGAATGAGACCTGGAACTGCCCCCCAAAAGCCACCGCTAACTAAGG CTCCGGTGCTGCCAATGGGTACTGAACTTCCGGCTGGCACTCTACCCAGC GAGCAGCTGCCACCTCTCCCAGGACCTTCTCTAACTCAGCCCCAGCAACC TCTAGAAGATCTTGATGCTCAGTTAAGAAGAACACTTAGTCCAGAGACTG TCGCTGTGGCATCAGCCCTTGGTCCTGTGTCCACGGTGTCTCCAGCAGCA GTTACAGAAGCAGGAGCACAGCCTCAGAAGGATGTTCCTCGAGGCGCAGA AGGCACTGCCCTAGCAGCTAGTTCAGGAGCTGGCGTTGTTAAGATGGGGC GATTTCAGGTTTCAGTTGCAATGGATGATGCCCAAAAACAAGGCAGAAAT AAGTCAGAAGATGCAAAGGCTGTTCGTTTTGAATCCAGCACTTCTGAATC CTCGGTGTTATCAAGCAGTAGCCCAGAGAGCACCCTGGTACAGCCTGAGC CCAGTGGGATAGCCATCCGTCGTATCTCTTTGGACATGCCAGACAGTGCC CACAAAGCTCCTGCCTCAGAGGCAAAGCCAGACACTGGACAGCCTACCAC AGTTGGGTGCATTCAGGTGACAACTACAGCAAACAAAGTGGGTCGTTTCT CTGTATCAAGAACTGAGGACAAGATTGCTGAGGCAAAAAAAGAGGGGCCA GTGGCACCTCCCCCTCTTACGGATTTGGAGCAAGCTGTTCTTCCTGCTGC AATACCAAAGAAAGACAAGCCTGAACTGTGTGAGCCTTCCCATCTGAATG GGCCATCTTCTGACTTGGAGGCCGCCTTTCTGAGTAGGGATATGGATGAG GGTTCAGGTAGTCCACACTCCCCGCCTCAGCTGTGTTCAAAGAGTCTTCC TATCCAGACTCTAAGTCAAAGCCTTAGTAATTCATTCAACTCCTCTTACA TGAGTAGTGACAATGAGTCTGACATTGAGGATGAAGACTTAAAGCTAGAG CTGCGAAGACTACGGGAAAAGCATCTTAAAGAGATTCAGGACCTGCAGAG TCGCCAGAAGCATGAAATCGAGTCTTTGTATACCAAACTGGGCAAGGTCC CCCCTGCTGTCATTATCCCCCCAGCTGCGCCCCTTTCAGGGAGAAGGAGG CGTCCCACTAAAAGCAAAGGAAGCAAATCCAGTCGTAGCAGTTCCTTGGG GAATAAAAGCCCCCAGCTTTCAGGTAATGTGTCTGGTCAGAGTGCAACTT CAGTCTTGCACCCCCAGCAGACCCTCCACCCTCCTGGCAGCATCCCAGAG ACCGGGCAGAACCAGCTGTTACAGCCCCTTAAGCCATCTCCCTCTAGTGA CAACCTCTATTCAGCCTTTACCAGTGATGGTGCCATTTCAGTACCAAGCC TTTCTGCTCCAGGTCAAGGGACCAGCAGCACAAACACCGTCGGGGGGACA GTGAGCAGTCAAGCTGCCCAGGCACAGCCCCCAGCCATGGCGTCCAGCAG GAAGGGCACGTTCACGGACGACCTGCACAAGCTAGTGGACAACTGGGCCC GGGATGCCATGAACCTTTCCGGCAGGAGAGGAAGCAAAGGACACATGAAC TACGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCGCCTGGGCAGCTGTG CATCTCCATGACCTCGAACCTGGGTGGCTCGGCCCCCATCTCTGCAGCGT CAGCTACCTCGCTAGGTCACTTCCCCAAGTCCATGTGCCCTCCACAGCAG TACGGTTTCCCAGCTGCCCCCTTCGGCACGCAGTGGAGTGGGACAGGCGG CCCAGCACCACAGCCACTTAGCCAGTTCCAGCCTGTGGGAACTGCCTCCT TGCAGAATTTCAACATCAGCAATTTGCAGAAATCCATCAGCAACCCCCCA GGTTCCAACCTGCGGACCACT |
Alignment |
Align specie | Pep length | Pep align begin | Pep align end | Mutaion type and postion |
Human | 2880 | 173 | 2880 | Insert and frame-shift 243-244:T-T^C, Insert and frame-shift 724-725:V-P^C, Insert and frame-shift 740-741:L-F^C, Insert and frame-shift 759-760:C-P^C |
Instructions: *** -> premature termination codon; # -> frame shift Alignment: Human pep sequence ---> |||||||||||||||||||||| Treeshrew pep sequence ---> Treeshrew cds sequence ---> GlySerLysGluGluProProProAlaArgSerGlySerGlyGlyGlySerAlaLysG ||| |||||||||||||||||||||||| |||||| ||||||| GlyGlyGlyGlyAspProProProAlaArgSerGlySerSerGlyGly---AlaLysG GGGGGAGGAGGGGACCCCCCGCCGGCCAGAAGCGGCAGCAGCGGCGGC---GCCAAGG luProGlnGluGluArgSerGlnGlnGlnAspAspIleGluGluLeuGluThrLysAl ||||||||||||||||||||||||||||||||||| |||||||||||||||||||| luProGlnGluGluArgSerGlnGlnGlnAspAspAsnGluGluLeuGluThrLysAl AGCCCCAGGAGGAGCGGAGCCAGCAGCAGGATGATAACGAGGAGCTGGAGACCAAGGC aValGlyMetSerAsnAspGlyArgPheLeuLysPheAspIleGluIleGlyArgGly |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| aValGlyMetSerAsnAspGlyArgPheLeuLysPheAspIleGluIleGlyArgGly CGTGGGCATGTCCAACGACGGCCGCTTTCTCAAGTTCGACATCGAGATCGGCAGGGGC SerPheLysThrValTyrLysGlyLeuAspThrGluThr-ThrValGluValAlaTrp |||||||||||| ||||||||||||||||||||| #|||||||||||||||||| SerPheLysThrGlyTyrLysGlyLeuAspThrGlu---#ThrValGluValAlaTrp TCCTTTAAGACGGGCTACAAAGGCCTGGACACCGAA---CACCGTGGAAGTGGCCTGG CysGluLeuGln >>>> Target Intron 1 >>>> AspArgLysLeuThrLy |||||||||||| 78104 bp ||||||||||||||||| CysGluLeuGln++ ++AspArgLysLeuThrLy TGCGAGCTGCAGgt agGATCGAAAGTTAACAAA sSerGluArgGlnArgPheLysGluGluAlaGluMetLeuLysGlyLeuGlnHisPro |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| sSerGluArgGlnArgPheLysGluGluAlaGluMetLeuLysGlyLeuGlnHisPro ATCTGAGAGGCAGAGATTTAAAGAAGAGGCTGAAATGCTAAAGGGTCTTCAGCATCCC AsnIleValArgPheTyrAspSerTrpGluSerThrValLysGlyLysLysCysIleV |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| AsnIleValArgPheTyrAspSerTrpGluSerThrValLysGlyLysLysCysIleV AATATTGTTCGATTCTACGATTCCTGGGAATCCACAGTAAAAGGAAAGAAGTGCATTG alLeuValThrGluLeuMetThrSerGlyThrLeuLys{Th} >>>> Target In ||||||||||||||||||||||||||||||||||||||{||} 6106 alLeuValThrGluLeuMetThrSerGlyThrLeuLys{Th}++ TTTTGGTGACTGAACTTATGACATCCGGAACACTTAAA{AC}gt tron 2 >>>> {r}TyrLeuLysArgPheLysValMetLysIleLysValLeuArg bp {|}|||||||||||||||||||||||||||||||||||||||||| ++{r}TyrLeuLysArgPheLysValMetLysIleLysValLeuArg ag{G}TACCTGAAAAGGTTTAAAGTGATGAAGATCAAAGTTCTAAGA SerTrpCysArgGlnIleLeuLysGlyLeuGlnPheLeuHisThrArgThrProProI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| SerTrpCysArgGlnIleLeuLysGlyLeuGlnPheLeuHisThrArgThrProProI AGCTGGTGCCGTCAGATTCTTAAAGGACTTCAGTTTCTTCATACTCGAACTCCACCTA leIleHisArgAspLeuLysCysAspAsnIlePheIleThrGlyProThrGlySerVa |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| leIleHisArgAspLeuLysCysAspAsnIlePheIleThrGlyProThrGlySerVa TTATTCACCGGGATCTTAAATGTGACAACATCTTTATCACTGGCCCTACAGGCTCAGT lLysIleGlyAspLeuGlyLeuAlaThrLeuLysArgAlaSerPheAlaLysSerVal |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lLysIleGlyAspLeuGlyLeuAlaThrLeuLysArgAlaSerPheAlaLysSerVal CAAGATTGGAGACCTTGGTCTGGCGACTCTGAAGCGGGCGTCTTTTGCCAAGAGTGTG Ile{G} >>>> Target Intron 3 >>>> {ly}ThrProGluPheMetAlaP |||{|} 7373 bp {||}||||||||||||||||||| Ile{G}++ ++{ly}ThrProGluPheMetAlaP ATA{G}gt ag{GT}ACCCCAGAGTTCATGGCAC roGluMetTyrGluGluLysTyrAspGluSerValAspValTyrAlaPheGlyMetCy |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| roGluMetTyrGluGluLysTyrAspGluSerValAspValTyrAlaPheGlyMetCy CTGAGATGTATGAAGAGAAATATGATGAATCTGTGGATGTCTATGCTTTTGGGATGTG sMetLeuGluMetAlaThrSerGluTyrProTyrSerGluCysGlnAsnAlaAlaGln |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| sMetLeuGluMetAlaThrSerGluTyrProTyrSerGluCysGlnAsnAlaAlaGln CATGCTTGAGATGGCTACATCTGAATATCCATACTCAGAGTGCCAAAATGCTGCGCAG IleTyrArgArgValThrSer >>>> Target Intron 4 >>>> GlyValLy ||||||||||||||||||||| 29707 bp |||||||| IleTyrArgArgValThrSer++ ++GlyValLy ATCTACCGTCGAGTGACGAGCgt agGGAGTAAA sProAlaSerPheAspLysValAlaIleProGluValLysGluIleIleGluGlyCys |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| sProAlaSerPheAspLysValAlaIleProGluValLysGluIleIleGluGlyCys GCCAGCCAGTTTTGACAAAGTTGCCATTCCTGAAGTCAAAGAAATTATTGAAGGATGC IleArgGlnAsnLysAspGlu{Ar} >>>> Target Intron 5 >>>> {g}T |||||||||||||||||||||{||} 2648 bp {|}| IleArgGlnAsnLysAspGlu{Ar}++ ++{g}T ATACGACAAAACAAAGATGAA{AG}gt ag{A}T yrSerIleLysAspLeuLeuAsnHisAlaPhePheGlnGluGluThrGlyValArgVa |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| yrSerIleLysAspLeuLeuAsnHisAlaPhePheGlnGluGluThrGlyValArgVa ACTCCATCAAAGACCTTTTGAACCATGCCTTCTTTCAGGAGGAAACAGGGGTACGGGT lGluLeuAlaGluGluAspAspGlyGluLysIleAlaIleLysLeuTrpLeuArgIle |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lGluLeuAlaGluGluAspAspGlyGluLysIleAlaIleLysLeuTrpLeuArgIle AGAATTAGCAGAAGAAGATGATGGAGAAAAGATAGCTATTAAATTATGGCTACGTATT GluAspIleLysLysLeuLysGlyLysTyrLysAspAsnGluAlaIleGluPheSerP |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GluAspIleLysLysLeuLysGlyLysTyrLysAspAsnGluAlaIleGluPheSerP GAAGACATTAAGAAACTAAAGGGAAAATACAAAGACAATGAAGCTATTGAGTTTTCCT heAspLeuGluArgAspValProGluAspValAlaGlnGluMet >>>> Target |||||||||||||||||||||||||||||||||||||||||||| 211 heAspLeuGluArgAspValProGluAspValAlaGlnGluMet++ TTGACTTGGAGAGAGATGTGCCAGAAGATGTTGCTCAAGAAATGgt Intron 6 >>>> ValGluSerGlyTyrValCysGluGlyAspHisLysThrMetA 2 bp ||||||||||||||||||||||||||||||||||||||||||| ++ValGluSerGlyTyrValCysGluGlyAspHisLysThrMetA agGTTGAGTCTGGGTATGTCTGTGAAGGTGATCACAAGACCATGG laLysAlaIleLysAspArgValSerLeuIleLysArgLysArgGluGlnArgGlnLe |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| laLysAlaIleLysAspArgValSerLeuIleLysArgLysArgGluGlnArgGlnLe CTAAAGCTATCAAAGATCGAGTGTCATTAATCAAGAGAAAACGGGAGCAGCGACAGTT uValArgGluGluGlnGluLysLysLysGlnGluGluSerSerLeuLysGlnGlnVal |||||||||||||||||||||| ||||||||||||||| ||||||||||||||| uValArgGluGluGlnGluLysArgLysGlnGluGluSerGlyLeuLysGlnGlnVal GGTACGCGAGGAGCAAGAAAAAAGAAAGCAGGAAGAGAGCGGTCTCAAACAGCAGGTG Glu<->GlnSerSerAlaSerGlnThrGlyIleLysGlnLeuProSerAlaSerThrG ||| ||||||||||||||||||||||||||||||||||||||||||||| |||| GluGlnGlnSerSerAlaSerGlnThrGlyIleLysGlnLeuProSerAlaGlyThrG GAACAGCAATCAAGTGCTTCCCAGACCGGAATCAAGCAGCTCCCGTCTGCTGGCACCG lyIleProThrAlaSerThrThrSerAlaSerValSerThrGlnValGluProGluGl |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lyIleProThrAlaSerThrThrSerAlaSerValSerThrGlnValGluProGluGl GCATCCCCACTGCTTCCACCACTTCAGCTTCAGTCTCTACACAGGTAGAACCTGAAGA uProGluAlaAspGlnHisGlnGlnLeuGlnTyrGlnGlnProSerIleSerValLeu ||||||||||||||||||||||||||||||||||||||||||| ||||||||| uProGluAlaAspGlnHisGlnGlnLeuGlnTyrGlnGlnProGlyAlaSerValLeu ACCTGAGGCAGATCAGCACCAACAACTGCAGTACCAGCAGCCTGGCGCATCTGTGTTA {S} >>>> Target Intron 7 >>>> {er}AspGlyThrValAspSerGlyG {|} 973 bp {||}|||||||||||||||||||||| {S}++ ++{er}AspGlyThrValAspSerGlyG {T}gt ag{CT}GATGGGACAGTTGACAGTGGTC lnGlySerSerValPheThrGluSerArgValSerSerGlnGlnThrValSerTyrGl |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lnGlySerSerValPheThrGluSerArgValSerSerGlnGlnThrValSerTyrGl AGGGATCTTCTGTCTTCACCGAATCTCGAGTTAGCAGCCAGCAGACAGTTTCATATGG ySerGlnHisGluGlnAlaHisSerThrGlyThrValProGlyHisIleProSerThr ||||||||||||| |||||||||||| ||||||||| ||| ySerGlnHisGluProThrHisSerThrGlySerMetProGlyHisThrAlaSerVal TTCCCAACATGAACCGACACATTCTACTGGCTCAATGCCAGGGCACACAGCTTCTGTC ValGlnAlaGlnSerGlnProHisGlyValTyrProProSerSerVal >>>> Tar |||||||||||||||||| ||||||||||||||||||||||||||| ValGlnAlaGlnSerGlnLeuHisGlyValTyrProProSerSerVal++ GTCCAAGCACAGTCTCAACTCCATGGGGTATATCCACCCTCAAGTGTGgt get Intron 8 >>>> ProArgArgGlyArgSerMetSerValCysVal-ProIl 3595 bp |||||||||||||||||||||||||||||||||#||| ++ProArgArgGlyArgSerMetSerValCysVal#ProMe agCCTCGTCGTGGCCGTAGCATGTCGGTTTGTGTTCCCCAT ePheLeuLeuLeuProLeuCysProAlaSerLeuProValLeu-PheHisProThrAl |||||||||||||||||||||||||||||| |||||||||#|||||||||||| tPheLeuLeuLeuProLeuCysProAlaSerPheProValLeu#PheHisProThrTh GTTTCTGCTGCTCCCTCTCTGTCCCGCATCTTTCCCAGTGCTCCTTCCACCCCACCAC aSerThrValCysThrSerPheSerPheProProProAspCys-ProGluGluThrPh ||| ||||||||| ||||||||||||||| ||||||#|||||||||||||| rSerAlaValCysThrIlePheSerPheProProSerAspCys#ProGluGluThrPh CAGTGCTGTCTGCACCATTTTCTCCTTCCCTCCTTCGGACTGCCCCTGAGGAAACTTT eAlaGluLysLeuSerLysAlaLeuGluSerValLeuProMetHisSerAlaSerGln |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| eAlaGluLysLeuSerLysAlaLeuGluSerValLeuProMetHisSerAlaSerGln TGCCGAAAAGCTCTCTAAAGCACTGGAGAGTGTCCTGCCTATGCACTCTGCCTCCCAG ArgLysHisArgArgSerSerLeuProSerLeuPheValSerThr >>>> Target ||||||||||||||||||||||||||||||||||||||||||||| 26 ArgLysHisArgArgSerSerLeuProSerLeuPheValSerThr++ CGCAAGCACCGACGCTCCAGCCTGCCTTCCCTCTTTGTCAGTACTgt Intron 9 >>>> ProGlnSerMetAlaHisProCysGlyGlyThrProThrTyr 35 bp |||||||||||||||||||||||||||||||||||||||||| ++ProGlnSerMetAlaHisProCysGlyGlyThrProThrTyr agCCTCAGTCCATGGCGCATCCGTGTGGGGGGACCCCAACCTAC ProGluSerGlnIlePhePheProThrIleHisGluArgProValSerPheSerProP |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ProGluSerGlnIlePhePheProThrIleHisGluArgProValSerPheSerProP CCAGAATCACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACCAC roProThrCysProProLysValAlaIleSerGlnArgArgLysSerThrSerPheLe |||||||||||||||||||| ||||||||||||||||||||||||||||||||||| roProThrCysProProLysIleAlaIleSerGlnArgArgLysSerThrSerPheLe CTCCTACCTGTCCACCGAAAATAGCCATTTCTCAGCGGCGTAAGAGCACCTCCTTCCT uGluAlaGlnThrHisHisPheGlnProLeuLeuArgThrValGlyGlnSerLeuLeu ||||||||||||| |||||||||||| |||||||||||||||||| |||||| uGluAlaGlnThrArgHisPheGlnProGlnLeuArgThrValGlyGlnAsnLeuLeu GGAAGCCCAAACTCGCCACTTCCAACCCCAGCTAAGGACTGTTGGCCAAAATCTTCTT ProProGlyGlySerProThrAsnTrpThrProGluAlaValValMetLeuGlyThrT |||||||||||||||||| ||||||||||||||||||||||||||||||||| ProProGlyGlySerProSerAsnTrpThrProGluAlaValValMetLeuGlyProS CCACCTGGTGGCAGCCCATCTAACTGGACACCAGAGGCCGTAGTTATGTTGGGTCCTT hrAlaSerArgValThrGlyGluSerCysGluIleGlnValHisProMetPheGluPr ||||||||||||||||||||| |||||||||||||||||||||||||||||||| erAlaSerArgValThrGlyGluProCysGluIleGlnValHisProMetPheGluPr CAGCCAGTAGAGTAACTGGAGAGCCATGTGAGATACAGGTCCATCCTATGTTTGAACC oSerGlnValTyrSerAspTyrArgProGlyLeuValLeuProGluGluAlaHisTyr | |||||||||||||||||||||||||||||||||||||||||||||||||||||| oThrGlnValTyrSerAspTyrArgProGlyLeuValLeuProGluGluAlaHisTyr AACTCAAGTCTACAGTGACTATAGACCTGGACTAGTACTTCCAGAAGAAGCTCATTAT PheIleProGlnGluAlaValTyrValAlaGlyValHisTyrGlnAlaArgValAlaG ||||||||| |||||| ||||||||||||||||||||| ||||||| LeuIleProGlnAspAlaValPheLeuAlaGlyValHisTyrGlnAlaGlnValAlaG CTTATTCCTCAGGATGCAGTGTTTCTAGCTGGGGTGCATTACCAGGCCCAGGTGGCAG luGlnTyrGluGlyIleProTyrAsnSerSerValLeuSerSerProMetLysGlnIl ||||||||||||||||||||||||||||| |||||||||||||||||||||||||| luGlnTyrGluGlyIleProTyrAsnSerProValLeuSerSerProMetLysGlnIl AACAGTATGAGGGCATCCCATACAACTCACCAGTACTGTCAAGTCCTATGAAACAGAT eProGluGlnLysProValGlnGlyGlyProThrSerSerSerValPheGluPhePro |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| eProGluGlnLysProValGlnGlyGlyProThrSerSerSerValPheGluPhePro ACCTGAACAGAAGCCAGTACAAGGGGGTCCTACCTCAAGTTCTGTCTTTGAATTTCCA SerGlyGlnAlaPheLeuValGlyHisLeuGlnAsnLeuArgLeuAspSerGlyLeuG |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| SerGlyGlnAlaPheLeuValGlyHisLeuGlnAsnLeuArgLeuAspSerGlyLeuG TCTGGACAGGCTTTCCTGGTAGGACACCTTCAGAATTTAAGATTAGATTCTGGATTGG lyProGlySerProLeuSerSerIleSerAlaProIleSerThrAspAlaThrArgLe ||||||||||||||||||||||||||||||||||| ||||||||||||||| || lyProGlySerProLeuSerSerIleSerAlaProLeuSerThrAspAlaThrCysLe GTCCAGGATCTCCCCTTTCTAGTATTTCTGCACCTCTCAGTACAGATGCTACATGTTT uLysPheHisProValPheValProHisSerAlaProAlaValLeuThrHisAsnAsn |||||||||||||||||||||||||||||||||||||||||||||||||||| ||| uLysPheHisProValPheValProHisSerAlaProAlaValLeuThrHisSerAsn GAAATTTCATCCTGTCTTTGTTCCTCATTCTGCACCCGCTGTGTTAACTCATAGCAAT GluSerArgSerAsnCysValPheGluPheHisValHisThrProSerSerSerSerG |||||||||||||||||||||||||||||||||||| ||||||||||||||||||| GluSerArgSerAsnCysValPheGluPheHisValGlnThrProSerSerSerSerG GAGAGCAGAAGCAACTGTGTATTTGAATTTCATGTTCAAACACCAAGCTCCTCTTCAG lyGluGlyGlyGlyIleLeuProGlnArgValTyrArgAsnArgGlnValAlaValAs |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lyGluGlyGlyGlyIleLeuProGlnArgValTyrArgAsnArgGlnValAlaValAs GAGAAGGAGGTGGAATTTTACCTCAGCGTGTGTACCGAAATCGACAGGTTGCGGTGGA pLeuAsnGlnGluGluLeuProProGlnSerValGlyLeuHisGlyTyrLeuGlnPro |||||||||||||||||||||||||||||||||||||||||||||| ||||||||| pLeuAsnGlnGluGluLeuProProGlnSerValGlyLeuHisGlyHisLeuGlnPro CTTAAACCAGGAAGAACTGCCTCCTCAATCGGTTGGATTACATGGCCACCTGCAGCCT ValThrGluGluLysHisAsnTyrHisAlaProGluLeuThrValSerValValGluP |||||||||||| ||||||||| ||||||||||||||| ||||||||||||| ValThrGluGluGlnHisAsnTyrGlnAlaProGluLeuThrIleSerValValGluP GTGACTGAAGAACAGCATAATTACCAGGCCCCAGAATTGACCATTTCTGTGGTAGAGC roIleGlyGlnAsnTrpProIleGlySerProGluTyrSerSerAspSerSerGlnIl || ||||||||||||||||||||||||||||||||||||||||||||||||||||| roValGlyGlnAsnTrpProIleGlySerProGluTyrSerSerAspSerSerGlnIl CTGTCGGACAGAACTGGCCAATAGGAAGCCCAGAATATTCCAGTGATTCCTCACAAAT eThrSerSerAspProSerAspPheGlnSerProProProThrGlyGlyAlaAlaAla |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| eThrSerSerAspProSerAspPheGlnSerProProProThrGlyGlyAlaAlaAla CACTTCTTCAGACCCCAGTGACTTTCAGTCACCTCCCCCTACAGGGGGAGCAGCAGCA ProPheGlySerAspValSerMetProPheIleHisLeuProGlnThrValLeuGlnG ||| |||||| |||||| ||||||||| |||||||||||||||||||||| ProLeuGlySerGluValSerLeuProPheIleArgLeuProGlnThrValLeuGlnG CCTCTTGGCTCTGAAGTCTCATTACCCTTTATCCGACTGCCTCAGACAGTGTTACAAG luSerProLeuPhePheCysPheProGlnGlyThrThrSerGlnGlnValLeuThrAl ||||||||||||||||||||||||||||||||||||||||||||||||||||| || luSerProLeuPhePheCysPheProGlnGlyThrThrSerGlnGlnValLeuSerAl AATCCCCACTCTTCTTCTGTTTTCCCCAAGGAACCACATCTCAGCAGGTCTTATCTGC aSerPheSerSerGlyGlySerAlaLeuHisProGln >>>> Target Intron |||||||||| |||||||||||||||||||||||| 3063 bp aSerPheSerProGlyGlySerAlaLeuHisProGln++ CTCATTTTCTCCAGGAGGATCTGCACTCCATCCACAGgt 10 >>>> AlaGlnGlyGlnSerGlnGlyGlnProSerSerSerSerLeuThrGlyV ||||||||||||||||||||||||||| ||||||||||||||||||| ++AlaGlnGlyGlnSerGlnGlyGlnProProSerSerSerLeuThrGlyV agGCGCAGGGGCAGAGCCAGGGGCAGCCACCCTCAAGCAGTTTAACAGGGG alSerSerSerGlnProIleGlnHisProGlnGln >>>> Target Intron 11 || |||||||||||||||||||||||||||||| 4609 bp alProSerSerGlnProIleGlnHisProGlnGln++ TTCCATCTTCCCAACCCATACAGCACCCTCAGCAGgt >>>> GlnGlnGlyIleGlnGlnThrAlaProProGlnGlnThrValGlnTyrSer ||||||||||||||||||||||||||||||||||||||||||||||||||| ++GlnGlnGlyIleGlnGlnThrAlaProProGlnGlnThrValGlnTyrSer agCAGCAGGGAATACAGCAGACAGCCCCTCCGCAACAGACAGTGCAGTATTCA LeuSerGlnThrSerThrSerSerGluAlaThrThrAlaGlnProValSerGlnProG ||||||||| ||| |||||| |||||||||||||||||||||| ProProGlnThrSerAlaSerAsnGluAlaProAlaAlaGlnProValSerGlnProG CCTCCCCAGACGTCAGCCTCCAATGAGGCCCCTGCTGCACAGCCAGTGAGTCAGCCTC lnAlaProGlnValLeuProGlnValSerAlaGlyLysGln >>>> Target Int || |||||||||||| |||||||||||||||||| 1359 lnThrThrGlnValLeuProProValSerAlaGlyLysGln++ AGACGACACAGGTCTTGCCTCCAGTATCAGCTGGAAAACAGgt ron 12 >>>> LeuProValSerGlnProValProThrIleGlnGlyGluProGln bp |||||||||||||||||| |||||||||||||||||||||||| ++LeuProValSerGlnProIleProThrIleGlnGlyGluProGln agCTTCCAGTTTCCCAGCCAATACCAACTATTCAAGGCGAACCTCAG IleProValAlaThrGlnProSerValValProValHisSerGlyAlaHisPheLeuP |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| IleProValAlaThrGlnProSerValValProValHisSerGlyAlaHisPheLeuP ATACCAGTCGCGACACAACCCTCAGTTGTTCCGGTTCACTCTGGTGCTCATTTCCTCC roValGlyGlnProLeuProThrProLeuLeuProGlnTyrProValSerGlnIlePr ||||||||||||||||| ||||||||||||||| |||||| || roValGlyGlnProLeuAlaProSerSerLeuProGlnTyrProLeuSerGlnThrPr CTGTGGGACAGCCACTTGCTCCTTCCTCACTCCCTCAGTACCCTCTCTCTCAAACTCC oIleSerThrProHisValSerThrAlaGlnThrGlyPheSerSerLeuProIleThr ||||||| ||| ||| |||||||||||||||||||||||| ||| oIleSerAlaProProMetProThrValGlnThrGlyPheSerSerLeuProValThr CATATCGGCTCCTCCCATGCCTACTGTGCAGACAGGTTTCTCATCCCTTCCTGTCACA MetAlaAlaGlyIleThrGlnProLeuLeuThrLeuAlaSerSerAlaThrThrAlaA ||||||||||||||| |||||||||||||||||||||||||||||| |||| MetAlaAlaGlyIleAsnGlnProLeuLeuThrLeuAlaSerSerAlaProAlaAlaA ATGGCAGCTGGCATTAACCAGCCTCTGCTCACCCTGGCTTCATCTGCTCCAGCAGCTG laIleProGlyValSerThrValValProSerGlnLeuProThrLeuLeuGlnProVa || |||||| ||| |||||| ||||||||||||||||||||||||||||| laValProGlyGlySerAlaValValAlaSerGlnLeuProThrLeuLeuGlnProVa CGGTCCCAGGGGGATCGGCTGTGGTTGCTAGTCAGCTGCCAACCCTTCTTCAGCCCGT lThrGlnLeuProSerGlnValHisProGlnLeuLeuGlnProAlaValGlnSerMet | ||||||||| ||| |||||||||||||||||||||||||||||||||||| lAlaGlnLeuProArgGlnThrHisProGlnLeuLeuGlnProAlaValGlnSerMet GGCTCAGCTGCCAAGGCAGACTCACCCACAGCTCCTACAGCCAGCAGTTCAGTCCATG GlyIleProAlaAsnLeuGlyGlnAlaAlaGluValProLeuSerSerGlyAspValL |||||||||||||||||||||||||||||||||||||||||| |||||||||||| GlyIleProAlaAsnLeuGlyGlnAlaAlaGluValProLeuProSerGlyAspValP GGAATACCAGCTAACCTTGGGCAAGCTGCTGAGGTGCCACTTCCCTCTGGAGATGTTC euTyrGln >>>> Target Intron 13 >>>> GlyPheProProArgLeuPr ||| 845 bp |||||||||||||||||||| roPheGln++ ++GlyPheProProArgLeuPr CGTTTCAGgt agGGCTTCCCACCTCGACTGCC oProGlnTyrProGlyAspSerAsnIleAlaProSerSerAsnValAlaSerValCys |||||||||||||||||||||||||||| ||||||||| ||||||||||||||| oProGlnTyrProGlyAspSerAsnIleValProSerSerSerValAlaSerValCys ACCACAATACCCAGGAGATTCAAACATTGTTCCCTCTTCCAGCGTGGCTTCTGTTTGC IleHisSerThrValLeuSerProProMetProThrGluValLeuAlaThrProGlyT ||||||||||||||||||||||||||||||||||||||| |||||||||||| | IleHisSerThrValLeuSerProProMetProThrGluAlaLeuAlaThrProAsnT ATCCATTCTACAGTCCTTTCCCCTCCCATGCCAACAGAAGCATTGGCTACACCAAATT yrPheProThrValValGlnProTyrValGluSerAsnLeuLeuValProMetGlyGl ||||||||||| ||||||||||||||||||||||||||| |||||| ||||| yrPheProThrMetValGlnProTyrValGluSerAsnLeuValValProValGlyGl ACTTTCCTACTATGGTGCAGCCTTATGTGGAATCAAATCTTGTGGTTCCTGTGGGCGG yValGlyGlyGlnValGlnValSerGlnProGlyGlySerLeuAla<->GlnAlaPro ||||||||||||||||||||||||||||||| ||||||||| ||||||||| yValGlyGlyGlnValGlnValSerGlnProAlaValSerLeuAlaGlnGlnAlaPro TGTAGGAGGACAGGTTCAAGTGTCTCAGCCAGCAGTGAGTTTAGCACAGCAAGCCCCC ThrThrSerSerGlnGlnAlaValLeuGlu >>>> Target Intron 14 >>>> ||| ||||||||| ||| |||||| 676 bp ThrAlaSerSerGlnProAlaIleLeuGlu++ ACTGCATCCTCCCAGCCAGCAATTCTGGAGgt SerThrGlnGlyValSerGlnValAlaProAlaGluProValAlaValAlaGlnTh |||||||||||||||||||||||||||||| ||||||||| ||||||||| ++SerThrGlnGlyValSerGlnValAlaProThrGluProValProValAlaGlnPr agAGTACTCAGGGAGTCTCTCAGGTGGCTCCTACAGAGCCAGTTCCGGTAGCACAGCC rGlnAlaThrGlnProThrThrLeuAlaSerSerValAsp{Se} >>>> Target ||| |||||| |||||| |||||| |||{||} 11 oGlnProProGlnProAlaThrLeuValSerSerIleAsp{Se}++ TCAGCCTCCCCAGCCTGCTACTTTGGTGTCCTCCATAGAT{AG}gt Intron 15 >>>> {r}AlaHisSerAspValAlaSerGlyMetSerAspGlyAsn 1 bp {|}||||||||||||||||||||||||||||||||||||||| ++{r}AlaHisSerAspValAlaSerGlyMetSerAspGlyAsn ag{T}GCACATTCCGATGTCGCCTCTGGTATGAGTGATGGCAAT GluAsnValProSerSerSerGlyArgHisGluGlyArgThrThrLysArgHisTyrA |||||||||||||||||| |||||||||||||||||| ||||||||||||| GluAsnValProSerSerGlyGlyArgHisGluGlyArgAlaProLysArgHisTyrA GAGAATGTCCCATCATCTGGTGGGAGACATGAAGGAAGAGCTCCAAAGCGGCACTATC rgLysSerValArgSerArgSerArgHisGluLysThrSerArgProLysLeuArgIl |||||||||||||||||||||||||||||||||||||| ||||||||||||||||| rgLysSerValArgSerArgSerArgHisGluLysThrAlaArgProLysLeuArgIl GAAAATCTGTACGGAGTCGCTCCCGGCATGAGAAGACGGCACGCCCCAAACTGAGAAT eLeuAsn >>>> Target Intron 16 >>>> ValSerAsnLysGlyAspArg ||||||| 1258 bp ||||||||||||||||||||| eLeuAsn++ ++ValSerAsnLysGlyAspArg TTTGAATgt agGTTTCAAATAAAGGAGACCGG ValValGluCysGlnLeuGluThrHisAsnArgLysMetValThrPheLysPheAspL |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ValValGluCysGlnLeuGluThrHisAsnArgLysMetValThrPheLysPheAspL GTAGTGGAATGTCAGTTAGAAACTCATAATCGGAAAATGGTCACGTTCAAATTTGACC euAspGlyAspAsnProGluGluIleAlaThrIleMet >>>> Target Intron |||||||||||||||||||||||||||||||||||||| 315 bp euAspGlyAspAsnProGluGluIleAlaThrIleMet++ TAGATGGTGATAACCCTGAGGAGATAGCAACGATTATGgt 17 >>>> ValAsnAsnAspPheIleLeuAlaIleGluArgGluSerPheValAsp |||||||||||||||||||||||||||||||||||||||||||||||| ++ValAsnAsnAspPheIleLeuAlaIleGluArgGluSerPheValAsp agGTGAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCGTTTGTGGAT GlnValArgGluIleIleGluLysAlaAspGluMetLeuSerGluAspValSerValG |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GlnValArgGluIleIleGluLysAlaAspGluMetLeuSerGluAspValSerValG CAAGTACGGGAAATCATTGAAAAAGCTGATGAAATGCTCAGTGAGGATGTCAGTGTGG luProGluGlyAspGlnGlyLeuGluSerLeuGlnGlyLysAspAspTyrGlyPheSe |||||||||||||||||||||||||| ||||||||||||||||||||||||||||| luProGluGlyAspGlnGlyLeuGluAsnLeuGlnGlyLysAspAspTyrGlyPheSe AACCCGAGGGTGACCAGGGATTGGAGAATCTACAGGGAAAGGATGACTATGGCTTTTC rGlySerGln >>>> Target Intron 18 >>>> LysLeuGluGlyGluPhe |||||||||| 201 bp |||||||||||||||||| rGlySerGln++ ++LysLeuGluGlyGluPhe AGGCTCTCAAgt agAAATTGGAAGGAGAGTTT LysGlnProIleProAlaSerSerMetProGlnGlnIle{G} >>>> Target In ||||||||| |||||||||||||||||||||||||||{|} 114 LysGlnProMetProAlaSerSerMetProGlnGlnIle{G}++ AAACAGCCAATGCCTGCGTCTTCCATGCCACAGCAAATA{G}gt tron 19 >>>> {ly}IleProThrSerSerLeuThrGlnValValHisSerAlaG bp {||} |||||||||||||||||||||||||||||||||| ++{ly}ValSerThrSerSerLeuThrGlnValValHisSerAlaG ag{GC}GTTTCTACCAGTTCTTTAACTCAAGTTGTTCATTCTGCTG lyArgArgPheIleValSerProValProGluSerArgLeuArgGluSerLysValPh ||||||||||||||||||||||||||||||||||||||||||||||||||||| || lyArgArgPheIleValSerProValProGluSerArgLeuArgGluSerLysLeuPh GAAGACGGTTTATAGTGAGTCCTGTGCCAGAAAGTCGATTACGAGAATCGAAACTTTT eProSerGluIleThrAspThr{V} >>>> Target Intron 20 >>>> {al | ||||||||| ||| {|} 1069 bp {|| eThrSerGluIleProAspLeu{V}++ ++{al CACCAGTGAAATACCAGATCTG{G}gt ag{TT }AlaAlaSerThrAlaGlnSerProGlyMetAsnLeuSerHisSerAlaSerSerLeu }|||||| ||| ||| |||||||||||||||||||||||||||||||||||| }AlaAlaCysThrSerGlnGlyProGlyMetAsnLeuSerHisSerAlaSerSerLeu }GCTGCCTGTACATCTCAAGGCCCTGGGATGAACTTGTCTCACTCTGCTTCATCCCTT SerLeuGlnGlnAlaPheSerGluLeuArgArgAlaGlnMetThrGluGlyProAsnT |||||||||||||||||||||||||||||| ||||||||||||||||||||| | SerLeuGlnGlnAlaPheSerGluLeuArgHisAlaGlnMetThrGluGlyProSerT AGTCTACAACAGGCCTTTTCCGAACTTAGACATGCCCAAATGACAGAAGGACCCAGTA hrAlaProProAsnPheSerHisThrGlyProThrPheProValValProProPheLe |||||||||||||||||||| ||||||||| ||||||||||||||||||||||| hrAlaProProAsnPheSerGlnThrGlyProAlaPheProValValProProPheLe CAGCACCTCCGAACTTCAGTCAGACGGGGCCAGCGTTTCCCGTTGTACCTCCCTTCTT uSerSerIleAlaGlyValProThrThrAlaAlaAla<-><->ThrAlaProValPro ||||||| |||||| |||||||||||| ||| ||| uSerSerValAlaGlyAlaProThrThrAlaSerAlaIleProSerValSerValSer GAGTAGTGTTGCTGGGGCCCCAACCACAGCATCCGCCATACCTTCAGTATCTGTCAGT AlaThrSerSerProProAsnAspIleSerThrSerValIleGlnSerGluValThrV |||||| ||| ||| |||||| |||||||||| AlaThrGlySerLeuLeuGlyAspValAlaThrSerAlaThrHisProGluValThrV GCCACAGGCAGTCTTCTGGGTGACGTTGCCACATCAGCAACTCATCCTGAGGTGACAG alProThrGluGluGlyIleAlaGlyValAlaThrSerThrGlyValValThrSerGl ||||| ||| ||| ||||||||| ||||||||||||||| ||| alProAlaGluAlaGlyMetAlaGlyValValThrSerThrGlyValIleThrProSe TACCCGCTGAGGCGGGGATGGCTGGAGTTGTCACTAGCACAGGTGTGATAACTCCAAG yGlyLeuProIleProProValSerGluSerProValLeuSerSerValValSerSer ||||||||| ||| ||||||||||||||| ||||||||||||||| rGlyLeuProValProLeuValSerGluSerProAlaProSerSerValValSerGly TGGTCTCCCTGTGCCACTTGTGTCTGAGTCGCCAGCACCCTCCAGTGTAGTCTCAGGC IleThrIleProAlaValValSerIleSerThrThrSerProSerLeuGlnValProT ||| |||||||||||| |||||| ||| |||| ThrThrValProAlaValValProVal---ProThrSerGlnTyrThrGlnAlaProT ACCACTGTACCTGCCGTGGTCCCGGTA---CCAACATCGCAGTACACTCAGGCCCCCA hrSer >>>> Target Intron 21 >>>> ThrSerGluIleValValSerSe ||||| 1323 bp ||| hrSer+- ++TyrThrGlnAlaProThrSerGl CGTCTgg agTACACTCAGGCCCCCACGTCTGG rThrAlaLeuTyrProSerValThrValSerAlaThrSerAlaSerAlaGlyGlySer ||| ||| |||||| ySerThrValSerSerThrGlyThrGlyProSerIleThrThrProAlaValGlySer AAGCACTGTTTCCAGTACTGGCACCGGTCCTTCCATAACAACCCCTGCAGTGGGCAGC ThrAlaThrProGlyProLysProProAlaValValSerGlnGlnAlaAlaGlySerT ||| ||| ||||||||||||||| |||||| ||||||||| AlaAlaAlaProAspAlaLysProProAlaValLeuProGlnGlnThrAlaGlySerS GCTGCTGCCCCCGACGCTAAGCCTCCAGCTGTGTTACCTCAGCAGACAGCAGGCAGTA hrThrValGly<->AlaThrLeuThrSerValSerThrThrThrSerPheProSerTh ||| ||| |||||| ||| |||||| ||| ||| erSerAlaGlyThrAlaAlaLeuThrAlaValProThrThrIleProPheSerSerVa GTAGTGCTGGGACAGCCGCCTTAACAGCGGTTCCTACCACCATTCCCTTCTCCAGTGT rAlaSerGlnLeuCysIleGlnLeuSerSerSerThrSerThrProThrLeuAlaGlu ||| ||||||||||||||||||||| ||||||||||||||| lAlaAlaHisProSerLeuGlnLeuSerSerSerThrSerAlaProThrLeuAlaGlu AGCTGCCCACCCGTCCCTGCAGCTTAGCAGCAGCACCTCAGCTCCTACTCTAGCTGAA ThrValValValSerAlaHisSerLeuAspLysThrSerHisSerSerThrThrGlyL ||||||||||||||||||||||||||||||||| ||||||||||||||| |||| ThrValValValSerAlaHisSerLeuAspLysValSerHisSerSerThrAlaGlyL ACAGTGGTGGTCAGTGCACACTCTCTAGATAAAGTGTCTCATAGCAGTACAGCTGGGC euAlaPheSerLeuSerAlaProSerSerSerSerSerProGlyAlaGlyValSerSe ||||| ||||||||||||||| |||||||||||||||||| ||| ||||| euAlaLeuSerLeuSerAlaPro---SerSerSerSerProGlyIleGlyLeuSerSe TGGCGCTGTCCCTCTCTGCTCCA---TCATCCTCTTCCCCTGGAATAGGGCTGTCTAG rTyrIleSerGlnProGlyGlyLeuHisProLeuValIleProSerValIleAlaSer | ||||||||| ||| |||||||||||| |||||| ||| ||| rSerValSerGlnProAlaGlyValHisProLeuValValProSerAlaIleSerSer TTCTGTTTCTCAGCCTGCTGGGGTACATCCTTTGGTGGTGCCATCAGCTATATCCTCT ThrProIleLeuProGlnAlaAlaGlyProThrSerThrProLeuLeuProGlnValP ||| ||| ||||||||| |||||||||||||||||||||||||||| AlaProVal---ProHisAlaAlaGlyLeuThrSerThrProLeuLeuProGlnValP GCTCCTGTT---CCCCATGCAGCAGGACTGACATCTACACCTTTACTGCCCCAGGTAC roSerIleProProLeuValGlnProValAlaAsnValProAlaValGlnGlnThrLe ||||| |||||||||||||||||||||||||||||||||||||||||||||||||| roSerValProProLeuValGlnProValAlaAsnValProAlaValGlnGlnThrLe CCAGTGTCCCCCCCTTGGTACAGCCTGTTGCCAATGTGCCTGCTGTGCAGCAGACTCT uIleHisSerGlnProGlnProAlaLeuLeuProAsnGlnProHisThrHisCysPro | |||||||||||||||||||||||||||||| ||||||||||||||||||||| uValHisSerGlnProGlnProAlaLeuLeuProSerGlnProHisThrHisCysPro GGTTCATAGTCAGCCACAGCCAGCTTTGCTTCCCAGCCAGCCCCATACTCACTGTCCT GluValAspSerAspThrGlnProLysAlaProGlyIleAspAspIleLysThrLeuG ||| ||| |||||||||||||||||||||||||||||||||||||||||||||| GluIleAspThrAspThrGlnProLysAlaProGlyIleAspAspIleLysThrLeuG GAAATTGACACCGATACTCAACCCAAAGCTCCTGGGATTGATGACATAAAGACCCTGG luGluLysLeuArgSerLeuPheSerGluHisSerSerSerGlyAlaGlnHisAlaSe |||||||||||||||||||||||||||||||||||||||||||||||||||||||| luGluLysLeuArgSerLeuPheSerGluHisSerSerSerGlyAlaGlnHisAlaPr AGGAAAAACTGCGATCTCTCTTCAGTGAACACAGCTCATCAGGAGCACAGCATGCCCC rValSerLeuGluThrSerLeuValIleGluSerThrValThrProGlyIleProThr |||||||||||||||||||||||| ||| |||||||||||| |||||| oValSerLeuGluThrSerLeuValValGluThrAlaValThrProGlyValProThr TGTCTCACTGGAGACGTCGTTGGTAGTAGAGACAGCTGTCACACCAGGCGTCCCAACC ThrAlaValAlaProSerLysLeuLeuThrSerThrThrSerThrCysLeuProProT |||||||||||||||||||||||| |||||||||||||||||||||||||||||| ThrAlaValAlaProSerLysLeuMetThrSerThrThrSerThrCysLeuProProA ACTGCTGTTGCACCAAGCAAGCTCATGACTTCCACTACAAGCACTTGCCTACCGCCTG hrAsnLeuProLeuGlyThrValAlaLeuProValThrProValValThrProGlyGl ||| ||||||||| ||||||||| |||||| ||||||||||| laSerLeuSerLeuGlyThrAlaGlyLeuProValIleProValGlyThrProGlyGl CAAGCTTGTCCTTGGGAACAGCTGGTTTGCCGGTTATACCAGTGGGCACACCTGGGCA nValSerThrProValSerThrThrThrSerGlyValLysProGlyThrAlaProSer | ||| ||||||||| ||| |||||| ||||||||||||||| nThrSerAlaProValSer---ThrAlaSerGlyMetArgProGlyThrAlaProGln AACTTCTGCTCCAGTGAGC---ACCGCATCAGGAATGAGACCTGGAACTGCCCCCCAA LysProProLeuThrLysAlaPro >>>> Target Intron 22 >>>> ValL |||||||||||||||||||||||| 1415 bp |||| LysProProLeuThrLysAlaPro++ ++ValL AAGCCACCGCTAACTAAGGCTCCGgt agGTGC euProValGlyThrGluLeuProAlaGlyThrLeuProSerGluGlnLeuProProPh ||||| |||||||||||||||||||||||||||||||||||||||||||||||| euProMetGlyThrGluLeuProAlaGlyThrLeuProSerGluGlnLeuProProLe TGCCAATGGGTACTGAACTTCCGGCTGGCACTCTACCCAGCGAGCAGCTGCCACCTCT eProGlyProSerLeuThrGln >>>> Target Intron 23 >>>> SerGln ||||||||||||||||||||| 3332 bp ||| uProGlyProSerLeuThrGln++ ++ProGln CCCAGGACCTTCTCTAACTCAGgt agCCCCAG GlnProLeuGluAspLeuAspAlaGlnLeuArgArgThrLeuSerProGluMetIleT ||||||||||||||||||||||||||||||||||||||||||||||||||| GlnProLeuGluAspLeuAspAlaGlnLeuArgArgThrLeuSerProGluThrValA CAACCTCTAGAAGATCTTGATGCTCAGTTAAGAAGAACACTTAGTCCAGAGACTGTCG hrValThrSerAlaValGly >>>> Target Intron 24 >>>> ProValSe ||| |||||| ||| 350 bp |||||||| laValAlaSerAlaLeuGly++ ++ProValSe CTGTGGCATCAGCCCTTGGTgt agCCTGTGTC rMetAlaAlaProThrAlaIleThrGluAlaGlyThrGlnProGlnLysGly{V} > | ||| ||| |||||||||||| |||||||||||| {|} rThrValSerProAlaAlaValThrGluAlaGlyAlaGlnProGlnLysAsp{V}++ CACGGTGTCTCCAGCAGCAGTTACAGAAGCAGGAGCACAGCCTCAGAAGGAT{G}gt >>> Target Intron 25 >>>> {al}SerGlnValLysGluGlyProValLeu 3323 bp {||} |||||| ||| +-{al}ProArgGlyAlaGluGlyThrAlaLeu at{TT}CCTCGAGGCGCAGAAGGCACTGCCCTA AlaThrSerSerGlyAlaGlyValPheLysMetGlyArgPheGln >>>> Target ||| |||||||||||||||||| |||||||||||||||||| 2 AlaAlaSerSerGlyAlaGlyValValLysMetGlyArgPheGln++ GCAGCTAGTTCAGGAGCTGGCGTTGTTAAGATGGGGCGATTTCAGgt Intron 26 >>>> ValSerValAlaAlaAspGlyAlaGlnLysGluGlyLysAs 218 bp |||||||||||| ||| ||||||||| ||| || ++ValSerValAlaMetAspAspAlaGlnLysGlnGlyArgAs agGTTTCAGTTGCAATGGATGATGCCCAAAAACAAGGCAGAAA nLysSerGluAspAlaLysSerValHisPheGluSerSerThrSerGluSerSerVal ||||||||||||||||||| ||| |||||||||||||||||||||||||||||| nLysSerGluAspAlaLysAlaValArgPheGluSerSerThrSerGluSerSerVal TAAGTCAGAAGATGCAAAGGCTGTTCGTTTTGAATCCAGCACTTCTGAATCCTCGGTG LeuSerSerSerSerProGluSerThrLeuValLysProGluProAsnGlyIleThrI ||||||||||||||||||||||||||||||||| ||||||||| |||||| | LeuSerSerSerSerProGluSerThrLeuValGlnProGluProSerGlyIleAlaI TTATCAAGCAGTAGCCCAGAGAGCACCCTGGTACAGCCTGAGCCCAGTGGGATAGCCA leProGlyIleSerSerAspValProGluSerAlaHisLysThrThrAlaSerGluAl || |||||| ||| ||| |||||||||||| ||||||||||| leArgArgIleSerLeuAspMetProAspSerAlaHisLysAlaProAlaSerGluAl TCCGTCGTATCTCTTTGGACATGCCAGACAGTGCCCACAAAGCTCCTGCCTCAGAGGC aLysSerAspThrGlyGlnProThrLysValGlyArgPheGlnValThrThrThrAla |||| |||||||||||||||||| |||||| |||||||||||||||||| aLysProAspThrGlyGlnProThrThrValGlyCysIleGlnValThrThrThrAla AAAGCCAGACACTGGACAGCCTACCACAGTTGGGTGCATTCAGGTGACAACTACAGCA AsnLysValGlyArgPheSerValSerLysThrGluAspLysIleThrAspThrLysL ||||||||||||||||||||||||||| ||||||||||||||| |||| AsnLysValGlyArgPheSerValSerArgThrGluAspLysIleAlaGluAlaLysL AACAAAGTGGGTCGTTTCTCTGTATCAAGAACTGAGGACAAGATTGCTGAGGCAAAAA ysGluGlyProValAlaSerProProPheMetAspLeuGluGlnAlaValLeuProAl ||||||||||||||||| |||||| |||||||||||||||||||||||||| ysGluGlyProValAlaProProProLeuThrAspLeuGluGlnAlaValLeuProAl AAGAGGGGCCAGTGGCACCTCCCCCTCTTACGGATTTGGAGCAAGCTGTTCTTCCTGC aValIleProLysLysGluLysProGluLeuSerGluProSerHisLeuAsnGlyPro | |||||||||||| |||||||||||| |||||||||||||||||||||||| aAlaIleProLysLysAspLysProGluLeuCysGluProSerHisLeuAsnGlyPro TGCAATACCAAAGAAAGACAAGCCTGAACTGTGTGAGCCTTCCCATCTGAATGGGCCA SerSerAspProGluAlaAlaPheLeuSerArgAspValAspAspGlySerGlySerP ||||||||| |||||||||||||||||||||||| ||| ||||||||||||| SerSerAspLeuGluAlaAlaPheLeuSerArgAspMetAspGluGlySerGlySerP TCTTCTGACTTGGAGGCCGCCTTTCTGAGTAGGGATATGGATGAGGGTTCAGGTAGTC roHisSerProHisGlnLeuSerSerLysSerLeuProSerGlnAsnLeuSerGlnSe ||||||||||| |||||| ||||||||||||||| ||| ||||||||||| roHisSerProProGlnLeuCysSerLysSerLeuProIleGlnThrLeuSerGlnSe CACACTCCCCGCCTCAGCTGTGTTCAAAGAGTCTTCCTATCCAGACTCTAAGTCAAAG rLeuSerAsnSerPheAsnSerSerTyrMetSerSerAspAsnGluSerAspIleGlu |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| rLeuSerAsnSerPheAsnSerSerTyrMetSerSerAspAsnGluSerAspIleGlu CCTTAGTAATTCATTCAACTCCTCTTACATGAGTAGTGACAATGAGTCTGACATTGAG AspGluAspLeuLysLeuGluLeuArgArgLeuArgAsp{Ly} >>>> Target I |||||||||||||||||||||||||||||||||||| {||} 474 AspGluAspLeuLysLeuGluLeuArgArgLeuArgGlu{Ly}++ GATGAAGACTTAAAGCTAGAGCTGCGAAGACTACGGGAA{AA}gt ntron 27 >>>> {s}HisLeuLysGluIleGlnAspLeuGlnSerArgGlnLysH bp {|}|||||||||||||||||||||||||||||||||||||||| ++{s}HisLeuLysGluIleGlnAspLeuGlnSerArgGlnLysH ag{G}CATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGC isGluIleGluSerLeuTyrThrLysLeuGlyLysValProProAlaValIleIlePr |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| isGluIleGluSerLeuTyrThrLysLeuGlyLysValProProAlaValIleIlePr ATGAAATCGAGTCTTTGTATACCAAACTGGGCAAGGTCCCCCCTGCTGTCATTATCCC oProAlaAlaProLeuSerGlyArgArgArgArgProThrLysSerLysGlySerLys |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| oProAlaAlaProLeuSerGlyArgArgArgArgProThrLysSerLysGlySerLys CCCAGCTGCGCCCCTTTCAGGGAGAAGGAGGCGTCCCACTAAAAGCAAAGGAAGCAAA SerSerArgSerSerSerLeuGlyAsnLysSerProGlnLeuSer{G} >>>> Tar |||||||||||||||||||||||||||||||||||||||||||||{|} SerSerArgSerSerSerLeuGlyAsnLysSerProGlnLeuSer{G}++ TCCAGTCGTAGCAGTTCCTTGGGGAATAAAAGCCCCCAGCTTTCA{G}gt get Intron 28 >>>> {ly}AsnLeuSerGlyGlnSerAlaAlaSerValLeuH 2732 bp {||}||| ||||||||||||||| |||||||||| ++{ly}AsnValSerGlyGlnSerAlaThrSerValLeuH ag{GT}AATGTGTCTGGTCAGAGTGCAACTTCAGTCTTGC isProGlnGlnThrLeuHisProProGlyAsnIleProGluSerGlyGlnAsnGlnLe ||||||||||||||||||||||||||||| ||||||||| |||||||||||||| isProGlnGlnThrLeuHisProProGlySerIleProGluThrGlyGlnAsnGlnLe ACCCCCAGCAGACCCTCCACCCTCCTGGCAGCATCCCAGAGACCGGGCAGAACCAGCT uLeuGlnProLeuLysProSerProSerSerAspAsnLeuTyrSerAlaPheThrSer |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| uLeuGlnProLeuLysProSerProSerSerAspAsnLeuTyrSerAlaPheThrSer GTTACAGCCCCTTAAGCCATCTCCCTCTAGTGACAACCTCTATTCAGCCTTTACCAGT AspGlyAlaIleSerValProSerLeuSerAlaProGlyGln{G} >>>> Target ||||||||||||||||||||||||||||||||||||||||||{|} 6 AspGlyAlaIleSerValProSerLeuSerAlaProGlyGln{G}++ GATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAA{G}gt Intron 29 >>>> {ly}ThrSerSerThrAsnThrValGlyAlaThrValAsnS 131 bp {||}|||||||||||||||||||||||| |||||| | ++{ly}ThrSerSerThrAsnThrValGlyGlyThrValSerS ag{GG}ACCAGCAGCACAAACACCGTCGGGGGGACAGTGAGCA erGlnAlaAlaGlnAlaGlnProProAlaMetThrSerSerArgLysGlyThrPheTh |||||||||||||||||||||||||||||||| ||||||||||||||||||||||| erGlnAlaAlaGlnAlaGlnProProAlaMetAlaSerSerArgLysGlyThrPheTh GTCAAGCTGCCCAGGCACAGCCCCCAGCCATGGCGTCCAGCAGGAAGGGCACGTTCAC rAspAspLeuHisLysLeuValAspAsnTrpAlaArgAspAlaMetAsnLeuSerGly |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| rAspAspLeuHisLysLeuValAspAsnTrpAlaArgAspAlaMetAsnLeuSerGly GGACGACCTGCACAAGCTAGTGGACAACTGGGCCCGGGATGCCATGAACCTTTCCGGC ArgArgGlySerLysGlyHisMetAsnTyrGlu >>>> Target Intron 30 > ||||||||||||||||||||||||||||||||| 821 bp ArgArgGlySerLysGlyHisMetAsnTyrGlu++ AGGAGAGGAAGCAAAGGACACATGAACTACGAGgt >>> GlyProGlyMetAlaArgLysPheSerAlaProGlyGlnLeuCysIleSerMe ||||||||||||||||||||||||||||||||||||||||||||||||||||| ++GlyProGlyMetAlaArgLysPheSerAlaProGlyGlnLeuCysIleSerMe agGGCCCTGGAATGGCAAGGAAGTTCTCTGCGCCTGGGCAGCTGTGCATCTCCAT tThrSerAsnLeuGlyGlySerAlaProIleSerAlaAlaSerAlaThrSerLeuGly |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| tThrSerAsnLeuGlyGlySerAlaProIleSerAlaAlaSerAlaThrSerLeuGly GACCTCGAACCTGGGTGGCTCGGCCCCCATCTCTGCAGCGTCAGCTACCTCGCTAGGT HisPheThrLysSerMetCysProProGlnGlnTyrGlyPheProAlaThrProPheG |||||| ||||||||||||||||||||||||||||||||||||||| ||||||| HisPheProLysSerMetCysProProGlnGlnTyrGlyPheProAlaAlaProPheG CACTTCCCCAAGTCCATGTGCCCTCCACAGCAGTACGGTTTCCCAGCTGCCCCCTTCG lyAlaGlnTrpSerGlyThrGlyGlyProAlaProGlnProLeuGlyGlnPheGlnPr || ||||||||||||||||||||||||||||||||||||||| ||||||||||| lyThrGlnTrpSerGlyThrGlyGlyProAlaProGlnProLeuSerGlnPheGlnPr GCACGCAGTGGAGTGGGACAGGCGGCCCAGCACCACAGCCACTTAGCCAGTTCCAGCC oValGlyThrAlaSerLeuGlnAsnPheAsnIleSerAsnLeuGlnLysSerIleSer |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| oValGlyThrAlaSerLeuGlnAsnPheAsnIleSerAsnLeuGlnLysSerIleSer TGTGGGAACTGCCTCCTTGCAGAATTTCAACATCAGCAATTTGCAGAAATCCATCAGC AsnProProGlySerAsnLeuArgThrThr |||||||||||||||||||||||||||||| AsnProProGlySerAsnLeuArgThrThr AACCCCCCAGGTTCCAACCTGCGGACCACT // |