| Basic information |
| Official Symbol | ISM2 |
| Description | isthmin 2 homolog (zebrafish) |
| Other Aliases | PSEC0137, TAIL1, THSD3 |
| Other Description | isthmin-2; thrombospondin and AMOP containing isthmin-like 1; thrombospondin and AMOP domain-containing isthmin-like protein 1; thrombospondin type-1 domain-containing protein 3; thrombospondin, type I domain-containing 3; thrombospondin, type I, domain containing 3 |
| Mutation type | premature termination 115:L->TGA, premature termination 119:T->TAG, premature termination 361:E->TAG |
| Location | Scaffold000528_1:2216991 2209946 - |
| Wiki | Wikipedia GeneCard |
| Sequence |
| ISM2 |
GGGTGAGCCCTGACCTAGGCCGCCCCGCCTTCCCTCTCTCCACAGGCCTC AGTCCCTCCAAATCCCAGGTCTTCGAAGGAAAGAGAGGAAGCACCACCAC ACCCCGGGACCCACCTGCAGGCAGGGCCGCGCCAGCATAGGCACTGGGCT GTCATGGAGCCAGGAACCCTGAGCCCGGGCAGGGCCACCCCTGCTGGGCC CCTGGAGGACACTCCCTTGCTGCTGGAGCTTCAGAAGCTGCCGGGATTGG CCAACACAGACTTGAGTGCCCCGAATCCCAACATCCAGGTGACCATCGAG GTGGTGGAGGACCCCCAGGCCGAGGTGGAAATGGACCTATTGGACGAGCC CAGCAATCACTGGCCCCAGGGCGCTCCCAACTGGCTGCCCACCAAGGAGC TCTTTTGGCCTCTCTTCTGGGGCTACCTGGAAGGCGAAGAAGGGGACACG AGGCTTGAGGGCAGGGCCCCAGGGGAAGAGGCGGAGGAGGAGGAAGAGGA GGACTACCTTGCAGAGTACAGTGAGGGCGAAGACCAGGAGGGCTACGATG AGGATGATGATGACAATGAGGAAGAACCATGGTCCAGTGGGGCCATGGAC AGTCAGGACCTGGGCTGGCCAGCCCCCAGAGATGGGACCTTCAAGGAGCT CGACAGCTATGACTATGAGCCTCAGGAGGAGTGGAGCCCCTGGTCTCCCT GCCCTGAGAGCTGCAGCAGCGGCAGCCAGCGGAGGACTCGGCCCTGTGGC TATGCCTGCACTGCCACCTAGTCCTGGGCCTGCGACCTGTCCCCCTGTCC CGGCGCCGAGGACGAGATCACCTTGGGCTTCCCCAGTGAGGGGTGGCAGC CCCTTACCCACAATGCTACGGACGTGCTTGGCTCAGATGTGGACAGCTGT GAGAAGTGGCTGAATTGCAAGAGCGACTTCCTGGCCAAGTACCTGAGCCA GGTGCTGCGGGACCTGCCCAGCTGCCCGTGCGCCTACCCGCTGGAGGCCG TGTACAGCGCCGTGAGCCTGCGGGACGAGCTCCAGGGCCGCAGCTTCCGG TGGCGGGACGCCAGCGGCCCGCGCGGGGGCCTGGGCGTGTACCCGCCCAC GGCGCGC |
| Alignment |
| Align specie | Pep length | Pep align begin | Pep align end | Mutaion type and postion |
| Human | 571 | 114 | 473 | Premature termination 115:L->TGA, Premature termination 119:T->TAG, Premature termination 361:E->TAG |
Instructions:
*** -> premature termination codon; # -> frame shift
Alignment:
Human pep sequence --->
||||||||||||||||||||||
Treeshrew pep sequence --->
Treeshrew cds sequence --->
GlyLeuAlaAsnThrThrLeuSerThrProAsnProAspThrGlnAlaSerAlaSerPro
||| ||| ||| ||| ||||||||| |||
Gly***AlaLeuThr***AlaAlaProProSerLeuSerProGlnAlaSerValProPro
GGGTGAGCCCTGACCTAGGCCGCCCCGCCTTCCCTCTCTCCACAGGCCTCAGTCCCTCCA
AspProArgProLeuArgGluGluGluGluAlaArgLeuLeuProArgThrHisLeuGln
|||||| ||| ||||||||| ||| ||||||||||||
AsnProArgSerSerLysGluArgGluGluAlaProProHisProGlyThrHisLeuGln
AATCCCAGGTCTTCGAAGGAAAGAGAGGAAGCACCACCACACCCCGGGACCCACCTGCAG
AlaGluLeuHisGlnHisGlyCysTrpThrValThrGluProAlaAlaLeuThrProGly
||| |||||| ||| ||| |||||| ||| ||||||
AlaGlyProArgGlnHisArgHisTrpAlaValMetGluProGlyThrLeuSerProGly
GCAGGGCCGCGCCAGCATAGGCACTGGGCTGTCATGGAGCCAGGAACCCTGAGCCCGGGC
AsnAlaThrProProArgThrGlnGluValThrProLeuLeuLeuGluLeuGlnLysLeu
||||||||| ||| ||||||||||||||||||||||||||||||
ArgAlaThrProAlaGlyProLeuGluAspThrProLeuLeuLeuGluLeuGlnLysLeu
AGGGCCACCCCTGCTGGGCCCCTGGAGGACACTCCCTTGCTGCTGGAGCTTCAGAAGCTG
ProGluLeuValHisAlaThrLeuSerThrProAsnProAspAsnGln >>>> Targe
||| ||| |||||| ||||||||| ||| 1
ProGlyLeuAlaAsnThrAspLeuSerAlaProAsnProAsnIleGln++
CCGGGATTGGCCAACACAGACTTGAGTGCCCCGAATCCCAACATCCAGgt
t Intron 1 >>>> ValThrIleLysValValGluAspProGlnAlaGluValSerI
772 bp ||||||||| |||||||||||||||||||||||||||
++ValThrIleGluValValGluAspProGlnAlaGluValGluM
agGTGACCATCGAGGTGGTGGAGGACCCCCAGGCCGAGGTGGAAA
leAspLeuLeuAlaGluProSerAsnProProProGlnAspThrLeuSerTrpLeuProA
||||||||| |||||||||||| |||||| |||||||||
etAspLeuLeuAspGluProSerAsnHisTrpProGlnGlyAlaProAsnTrpLeuProT
TGGACCTATTGGACGAGCCCAGCAATCACTGGCCCCAGGGCGCTCCCAACTGGCTGCCCA
la<-><-><->LeuTrpSerPheLeuTrpGlyAspTyrLysGlyGluGluLysAsp<-><
||| |||||| ||||||||| |||
hrLysGluLeuPheTrpProLeuPheTrpGlyTyrLeuGluGlyGluGluGlyAspThrA
CCAAGGAGCTCTTTTGGCCTCTCTTCTGGGGCTACCTGGAAGGCGAAGAAGGGGACACGA
-><-><-><->ArgAlaProGlyGluLysGlyGluGluLysGluGluAspGluAspTyrP
||||||||||||||| |||||| |||||| |||||||||
rgLeuGluGlyArgAlaProGlyGluGluAlaGluGlu---GluGluGluGluAspTyrL
GGCTTGAGGGCAGGGCCCCAGGGGAAGAGGCGGAGGAG---GAGGAAGAGGAGGACTACC
roSerGluAspIleGluGlyGluAspGlnGlu<-><->AspLysGluGluAspGluGluG
||| |||||||||||||||||| ||| ||| |
euAlaGluTyrSerGluGlyGluAspGlnGluGlyTyrAspGluAspAspAspAspAsnG
TTGCAGAGTACAGTGAGGGCGAAGACCAGGAGGGCTACGATGAGGATGATGATGACAATG
luGlnAlaLeuTrpPheAsnGlyThrThrAspAsnTrpAspGlnGlyTrpLeuAlaProG
|| ||| ||| ||| ||| |||||| ||||||
luGluGluProTrpSerSerGlyAlaMetAspSerGlnAspLeuGlyTrpProAlaProA
AGGAAGAACCATGGTCCAGTGGGGCCATGGACAGTCAGGACCTGGGCTGGCCAGCCCCCA
lyAspTrpValPheLysAspSerValSerTyr{A} >>>> Target Intron 2 >>
||| |||||| ||||||{|} 2760 bp
rgAspGlyThrPheLysGluLeuAspSerTyr{A}++
GAGATGGGACCTTCAAGGAGCTCGACAGCTAT{G}gt
>> {sp}TyrGluProGlnLysGluTrpSerProTrpSerProCysSerGlyAsnCysS
{||}|||||||||||| |||||||||||||||||||||||| ||||
++{sp}TyrGluProGlnGluGluTrpSerProTrpSerProCysProGluSerCysS
ag{AC}TATGAGCCTCAGGAGGAGTGGAGCCCCTGGTCTCCCTGCCCTGAGAGCTGCA
erThrGlyLysGlnGlnArgThrArgProCysGlyTyrGlyCysThrAlaThrGluThrA
|| ||| ||| ||||||||||||||||||||| ||||||||||||
erSerGlySerGlnArgArgThrArgProCysGlyTyrAlaCysThrAlaThr***SerT
GCAGCGGCAGCCAGCGGAGGACTCGGCCCTGTGGCTATGCCTGCACTGCCACCTAGTCCT
rgThrCysAspLeuProSerCysPro{G} >>>> Target Intron 3 >>>> {l
||||||||| ||||||{|} 267 bp {|
rpAlaCysAspLeuSerProCysPro{G}++ ++{l
GGGCCTGCGACCTGTCCCCCTGTCCC{G}gt ag{G
y}ThrGluAspLysAspThrLeuGlyLeuProSerGluGluTrpLysLeuLeuAlaArgA
|} |||||| ||||||||| ||||||||| ||| ||| |
y}AlaGluAspGluIleThrLeuGlyPheProSerGluGlyTrpGlnProLeuThrHisA
C}GCCGAGGACGAGATCACCTTGGGCTTCCCCAGTGAGGGGTGGCAGCCCCTTACCCACA
snAlaThrAspMetHisAspGln{A} >>>> Target Intron 4 >>>> {sp}V
||||||||||| {|} 1140 bp {||}|
snAlaThrAspValLeuGlySer{A}++ ++{sp}V
ATGCTACGGACGTGCTTGGCTCA{G}gt ag{AT}G
alAspSerCysGluLysTrpLeuAsnCysLysSerAspPheLeuIleLysTyrLeuSerG
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
alAspSerCysGluLysTrpLeuAsnCysLysSerAspPheLeuAlaLysTyrLeuSerG
TGGACAGCTGTGAGAAGTGGCTGAATTGCAAGAGCGACTTCCTGGCCAAGTACCTGAGCC
lnMetLeuArgAspLeuProSerCysProCysAlaTyrProLeuGluAlaMetAspSerP
|| ||||||||||||||||||||||||||||||||||||||||||||| |||
lnValLeuArgAspLeuProSerCysProCysAlaTyrProLeuGluAlaValTyrSerA
AGGTGCTGCGGGACCTGCCCAGCTGCCCGTGCGCCTACCCGCTGGAGGCCGTGTACAGCG
roValSerLeuGlnAspGluHisGlnGlyArgSerPheArgTrpArgAspAlaSerGlyP
||||||||| |||||| |||||||||||||||||||||||||||||||||||||
laValSerLeuArgAspGluLeuGlnGlyArgSerPheArgTrpArgAspAlaSerGlyP
CCGTGAGCCTGCGGGACGAGCTCCAGGGCCGCAGCTTCCGGTGGCGGGACGCCAGCGGCC
roArgGluArgLeuAspIleTyrGlnProThrAlaArg
||||| ||| ||| ||||||||||||
roArgGlyGlyLeuGlyValTyrProProThrAlaArg
CGCGCGGGGGCCTGGGCGTGTACCCGCCCACGGCGCGC
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