Basic information
Official Symbol DROSHA
Descriptiondrosha, ribonuclease type III
Other AliasesETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN
Other DescriptionRNase III; drosha, double-stranded RNA-specific endoribonuclease; nuclear RNase III Drosha; p241; protein Drosha; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; ribonuclease 3; ribonuclease III, nuclear; ribonuclease type III, nuclear
Mutation typeInsert and frame-shift 348-349:L-E^CCAC
LocationScaffold000237_1:2058193 1914231 -

  ±  bp flank
WikiWikipedia GeneCard
Sequence
DROSHA
CATAGAATGTCGTTCCACCCAGGACGAGGCTGTCCCCGAGGACGAGGAGG
ACATGGAGCCAGACCCTCAGCCCCAGCCTTCAGGCCCCAAAATCTGAGAC
TTCTTCATCCTCAGCAGCCTCCTGTGCAATATCAATACGAGCCTCCAAGT
GCCCCTTCCACCACGTTCTCGAACTCTCCAGCCCCCAATTTTCTGCCTCC
ACGACCAGACTTTGTCCCTTTCCCTCCACCCATGCCTCCATCAGCACAAG
GCCCTCTTCCCCCCTGCCCAATCCGGCCCCCTTTCCCCAACCACCAGATG
AGGCACCCCTTTCCGGTGCCTCCCTGTTTTCCTCCCATGCCACCTCCAAT
GCCCTGTCCCAACAACCCCCCAGTCCCTGGAACGCCTCCTGGACAAGGCA
GTTTCCCCTTCATGATGCCCCCTCCCTCTGTGCCCCATCCTCCACCCCCT
CCAGTCATGCCACAGCAGGTTAATTATCAGTACCCTCCGGGATACTCACA
CCACAATTTCCCACCTCCCAGTTTTAATAGTTTCCAGAACAGCCCCAGTT
CTTTCCCACCCAGTGCTAGTAACAGCAGTAGCCCTCACTTTAGGCACCTT
GCTCCTTACCCGCTCCCAAAGGCTACCAGTGAGAGAAGGTCTCCAGAAAG
GCTCAAACATTATGACGATCACAGGCACCGAGATCACAGTCATGGGCGAG
GCGAGAAGCGCCGGTCCTTGGATCGGCGGGAGCGAGGTCGCAGTCCCAAC
AGGAGAAGGCAAGACAGTCGCTACAGATCCGATTACGAACGTGGGAGAAC
GCCCTCTCGCCACCGCAGCTATGAGCGGAGCAGAGAGCGGGAACGGGAGA
GACACAGGCATCGAGACAACCGAAGATCACCCTCTCTGGAAAGGTCCTAC
AAAAAAGAGTATAAGAGATCTGGAAGGAGTAATGGTTTACCAGCTATTCC
TGAACCTGCTGGATGCACATCAGAATTACCTGGGGAGATTATTAAAAATA
CAGATTCTTGCCCCTCCCCCCCTGCCGAGATTGTGAATCATCGCTCCCCA
AGTAGGGAGAAGAAGCGAGCTCGTTGGGAGGAAGAAAAAGACAGGTGGAG
TGACAACCAGAGCTCCGGCAAAGAGAAAAACTATACCTCCATCAAGGAAA
AGGAGCCGGAGGAGACGCTGACTGACAAAAATGAGGAGGAGGAAGAAGAA
CTACTGAAGCCCGTGTGGATCCGATGTACCCATTCAGAAAACTACTATTC
CAGTGACCCCATGGATCAGGTGGGAGATTCTACTGTAGTTGGAACAAGTA
GACTTCGTGACTTGTATGACAAATTTGAAGAAGAGTTAGGGAGCAGGCAA
GAGAAGGCTAAGGCCGCTAGACCTCCGTGGGAACCTCCGAAAACGAAGCT
GGACGAGGATTTAGAGAGTTCCAGTGAGTCCGAATGTGAGTCCGATGAAG
ACAGCACCTGTTCTAGCAGCTCAGATTCTGAAGTTTTTGACGTTATTGCA
GAAATCAAACGCAAAAAGGCTCATCCTGACCGACTTCACGATGAACTTTG
GTACAATGACCCAGGCCAGATGAATGACGGGCCACTCTGCAAATGCAGCG
CAAAAGCGAGACGCACAGGAATTAGGCACAGCATTTATCCCGGAGAAGAG
AACATCAAGCCTTGTCGTCCGATGACCAACAATGCTGGCAGACTTTTCCA
CTATCGGATTACGGTTTCCCCCCCTACAAACTTTTTAACTGACAGGCCAA
CCGTTATAGAATATGATGATCATGAGTATATCTTCGAAGGATTTTCTATG
TTTGCACATGCCCCCCTGACCAATATTCCATTGTGTAAAGTTATTAGATT
CAACATAGACTACACAATTCATTTCATTGAAGAGATGATGCCTGAGAATT
TTTGTGTAAAAGGGCTTGAACTCTTTTCATTGTTCCTATTCAGAGATATT
TTGGAATTATACGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGTCC
TCCTTGCTGCCCAAGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTC
CAGATGGAGGAAAGGAAGTTCTGTCCATGCACCAGATCCTCCTCTACTTA
CTCCGGTGTAGCAAAGCACTGGTCCCTGAGGAGGAGATTGCCAATATGCT
GCAGTGGGAAGAACTTGAGTGGCAGAAATATGCCGAGGAATGCAAAGGCA
TGATCGTGACCAACCCTGGGACGAAACCAAGTTCTGTCCGCATTGATCAA
CTGGATCGTGAACAGTTCAACCCTGATGTGATTACTTTCCCAATTATTGT
CCACTTTGGGATACGCCCTGCACAGTTGAGTTATGCAGGAGACCCACAGT
ATCAGAAACTGTGGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCAAAT
AGTCCCAAAGTCAAACAAACTGACAAGCAAAAGCTGGCACAGAGGGAGGA
AGCACTCCAAAAAATACGACAGAAGAATACAATGAGGCGGGAGGTAACCG
TGGAGCTAAGTAGCCAGGGATTCTGGAAAACCGGCATCCGTTCTGATGTC
TGCCAGCATGCCATGATGCTGCCTGTTTTGACCCATCATATCCGCTACCA
CCAATGCCTAATGCATCTGGACAAGCTGATAGGATATACTTTCCAAGATC
GTTGTCTGTTACAGTTGGCCATGACTCATCCAAGCCATCATTTAAATTTT
GGAATGAATCCTGATCACGCCAGGAATTCTTTATCTAACTGTGGAATTCG
GCAGCCCAAATACGGAGACAGAAAAGTTCATCACATGCACATGCGTAAGA
AAGGAATTAACACCTTAATAAATATTATGTCACGCCTTGGACAAGATGAC
CCAACTCCTTCAAGGATTAATCACAATGAAAGACTGGAGTTCCTGGGTGA
TGCTGTGGTTGAATTTCTGACCAGTGTCCATTTGTACTATTTGTTTCCTA
GTCTGGAAGAAGGAGGATTAGCAACCTACCGGACTGCCATTGTTCAGAAT
CAGCACCTTGCCATGCTAGCAAAGAAACTTGAACTGGATCGATTTATGCT
ATACGCTCATGGGCCTGACCTTTGTAGAGAATCGGACCTTCGACATGCAA
TGGCTAATTGTTTTGAAGCATTAATAGGAGCTGTTTACTTGGAGGGAAGC
CTGGAGGAAGCCAAGCAGCTATTTGGACGCTTACTCTTTAATGATCCGGA
CCTGCGCGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAG
AACCAAATACTGATCGACAACTTATTGAGACTTCTCCAGTTCTGCAGAAA
CTTACTGAGTTCGAAGAAGCAATTGGAGTGATTTTTACTCATGTTCGACT
TCTGGCAAGGGCATTCACTTTGAGGACTGTGGGATTTAACCATCTGACCT
TAGGCCACAACCAGAGGATGGAATTCCTAGGTGACTCCATAATGCAGCTG
GTAGCCACAGAGTACTTATTTATTCATTTCCCAGATCATCATGAAGGACA
CTTAACTTTGTTGCGAAGCTCTTTGGTGAATAACAGGACTCAAGCCAAGG
TAGCAGAGGAGCTGGGCATGCAGGAATATGCCATAACGAACGACAAGACC
AAGAGACCCGTGGCCCTTCGGACCAAGACCTTGGCGGACCTCTTGGAATC
GTTTATTGCAGCATTGTACATTGATAAGGATTTGGAATATGTTCATACTT
TCATGAATGTCTGCTTCTTTCCACGATTAAAAGAGTTCATTTTGAATCAG
GATTGGAATGACCCCAAATCCCAGCTGCAGCAGTGTTGCCTGACACTGAG
GACAGAAGGCAAAGAGCCAGATATTCCTCTGTACAAGACTCTGCAGACCG
TGGGCCCGTCCCATGCTAGAACTTACACTGTGGCTGTGTATTTCAAGGGA
GAAAGAATAGGCTGTGGGAAAGGACCAAGCATTCAGCAAGCAGAAATGGG
AGCTGCAATGGATGCACTTGAAAAATATAACTTTCCCCAGATGGCCCATC
AGAAGCGGTTCATTGAACGGAAATACAGACAGGAGTTGAAGGAGATGAGG
TGGGAAAGAGAGCATCAAGAGAGAGAGCCTGATGAGACCGAGGACATGAA
GAAG

Alignment
Align speciePep lengthPep align beginPep align endMutaion type and postion
Human137481374Insert and frame-shift 348-349:L-E^CCAC
 Instructions:
 *** -> premature termination codon;  # -> frame shift 

 Alignment:
 Human     pep sequence --->
 ||||||||||||||||||||||
 Treeshrew pep sequence --->
 Treeshrew cds sequence --->
  
 HisArgMetSerPheHisProGlyArgGlyCysProArgGlyArgGlyGlyHisGlyA
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 HisArgMetSerPheHisProGlyArgGlyCysProArgGlyArgGlyGlyHisGlyA
 CATAGAATGTCGTTCCACCCAGGACGAGGCTGTCCCCGAGGACGAGGAGGACATGGAG

 laArgProSerAlaProSerPheArgProGlnAsnLeuArgLeuLeuHisProGlnGl
 |||||||||||||||||   ||||||||||||||||||||||||||||||||||||||
 laArgProSerAlaProAlaPheArgProGlnAsnLeuArgLeuLeuHisProGlnGl
 CCAGACCCTCAGCCCCAGCCTTCAGGCCCCAAAATCTGAGACTTCTTCATCCTCAGCA

 nProProValGlnTyrGlnTyrGluProProSerAlaProSerThrThrPheSerAsn
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 nProProValGlnTyrGlnTyrGluProProSerAlaProSerThrThrPheSerAsn
 GCCTCCTGTGCAATATCAATACGAGCCTCCAAGTGCCCCTTCCACCACGTTCTCGAAC

 SerProAlaProAsnPheLeuProProArgProAspPheValProPheProProProM
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 SerProAlaProAsnPheLeuProProArgProAspPheValProPheProProProM
 TCTCCAGCCCCCAATTTTCTGCCTCCACGACCAGACTTTGTCCCTTTCCCTCCACCCA

 etProProSerAlaGlnGlyProLeuProProCysProIleArgProProPheProAs
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 etProProSerAlaGlnGlyProLeuProProCysProIleArgProProPheProAs
 TGCCTCCATCAGCACAAGGCCCTCTTCCCCCCTGCCCAATCCGGCCCCCTTTCCCCAA

 nHisGlnMetArgHisProPheProValProProCysPheProProMetProProPro
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 nHisGlnMetArgHisProPheProValProProCysPheProProMetProProPro
 CCACCAGATGAGGCACCCCTTTCCGGTGCCTCCCTGTTTTCCTCCCATGCCACCTCCA

 MetProCysProAsnAsnProProValProGlyAlaProProGlyGlnGlyThrPheP
 |||||||||||||||||||||||||||||||||   |||||||||||||||   ||||
 MetProCysProAsnAsnProProValProGlyThrProProGlyGlnGlySerPheP
 ATGCCCTGTCCCAACAACCCCCCAGTCCCTGGAACGCCTCCTGGACAAGGCAGTTTCC

 roPheMetMetProProProSerMetProHisProProProProProValMetProGl
 |||||||||||||||||||||||   ||||||||||||||||||||||||||||||||
 roPheMetMetProProProSerValProHisProProProProProValMetProGl
 CCTTCATGATGCCCCCTCCCTCTGTGCCCCATCCTCCACCCCCTCCAGTCATGCCACA

 nGlnValAsnTyrGlnTyrProProGlyTyrSerHisHisAsnPheProProProSer
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 nGlnValAsnTyrGlnTyrProProGlyTyrSerHisHisAsnPheProProProSer
 GCAGGTTAATTATCAGTACCCTCCGGGATACTCACACCACAATTTCCCACCTCCCAGT

 PheAsnSerPheGlnAsnAsnProSerSerPheLeuProSerAlaAsnAsnSerSerS
 ||||||||||||||||||   ||||||||||||   |||||||||   ||||||||||
 PheAsnSerPheGlnAsnSerProSerSerPheProProSerAlaSerAsnSerSerS
 TTTAATAGTTTCCAGAACAGCCCCAGTTCTTTCCCACCCAGTGCTAGTAACAGCAGTA

 erProHisPheArgHisLeuProProTyrProLeuProLysAlaProSerGluArgAr
 ||||||||||||||||||||   |||||||||||||||||||||   |||||||||||
 erProHisPheArgHisLeuAlaProTyrProLeuProLysAlaThrSerGluArgAr
 GCCCTCACTTTAGGCACCTTGCTCCTTACCCGCTCCCAAAGGCTACCAGTGAGAGAAG

 gSerProGluArgLeuLysHisTyrAspAspHisArgHisArgAspHisSerHisGly
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 gSerProGluArgLeuLysHisTyrAspAspHisArgHisArgAspHisSerHisGly
 GTCTCCAGAAAGGCTCAAACATTATGACGATCACAGGCACCGAGATCACAGTCATGGG

 ArgGlyGluArgHisArgSerLeuAspArgArgGluArgGlyArgSerProAspArgA
 |||||||||      ||||||||||||||||||||||||||||||||||||   ||||
 ArgGlyGluLysArgArgSerLeuAspArgArgGluArgGlyArgSerProAsnArgA
 CGAGGCGAGAAGCGCCGGTCCTTGGATCGGCGGGAGCGAGGTCGCAGTCCCAACAGGA

 rgArgGlnAspSerArgTyrArgSerAspTyrAspArgGlyArgThrProSerArgHi
 ||||||||||||||||||||||||||||||||   |||||||||||||||||||||||
 rgArgGlnAspSerArgTyrArgSerAspTyrGluArgGlyArgThrProSerArgHi
 GAAGGCAAGACAGTCGCTACAGATCCGATTACGAACGTGGGAGAACGCCCTCTCGCCA

 sArgSerTyrGluArgSer{Ar}  >>>> Target Intron 1 >>>>  {g}Glu
 |||||||||||||||||||{||}           4819 bp           {|}|||
 sArgSerTyrGluArgSer{Ar}++                         ++{g}Glu
 CCGCAGCTATGAGCGGAGC{AG}gt                         ag{A}GAG

 ArgGluArgGluArgHisArgHisArgAspAsnArgArgSerProSerLeuGluArgS
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 ArgGluArgGluArgHisArgHisArgAspAsnArgArgSerProSerLeuGluArgS
 CGGGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCACCCTCTCTGGAAAGGT

 erTyrLysLysGluTyrLysArgSerGly{Ar}  >>>> Target Intron 2 >>
 |||||||||||||||||||||||||||||{||}           7526 bp       
 erTyrLysLysGluTyrLysArgSerGly{Ar}++                       
 CCTACAAAAAAGAGTATAAGAGATCTGGA{AG}gt                       

 >>  {g}SerTyrGlyLeuSerValValProGluProAlaGlyCysThrProGluLeu
     {|}|||   ||||||         |||||||||||||||||||||   ||||||
   ++{g}SerAsnGlyLeuProAlaIleProGluProAlaGlyCysThrSerGluLeu
   ag{G}AGTAATGGTTTACCAGCTATTCCTGAACCTGCTGGATGCACATCAGAATTA

 ProGlyGluIleIleLysAsnThrAspSerTrp<->AlaProProLeu----GluIle
 ||||||||||||||||||||||||||||||         ||||||   ####||||||
 ProGlyGluIleIleLysAsnThrAspSerCysProSerProProAla####GluIle
 CCTGGGGAGATTATTAAAAATACAGATTCTTGCCCCTCCCCCCCTGCCCCACGAGATT

 ValAsn{Hi}  >>>> Target Intron 3 >>>>  {s}ArgSerProSerArgG
 ||||||{||}            232 bp           {|}||||||||||||||||
 ValAsn{Hi}++                         ++{s}ArgSerProSerArgG
 GTGAAT{CA}gt                         ag{T}CGCTCCCCAAGTAGGG

 luLysLysArgAlaArgTrpGluGluGluLysAspArgTrpSerAspAsnGlnSerSe
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 luLysLysArgAlaArgTrpGluGluGluLysAspArgTrpSerAspAsnGlnSerSe
 AGAAGAAGCGAGCTCGTTGGGAGGAAGAAAAAGACAGGTGGAGTGACAACCAGAGCTC

 rGlyLysAspLysAsnTyrThrSerIleLysGluLysGluProGluGluThrMetPro
 |||||||   ||||||||||||||||||||||||||||||||||||||||||      
 rGlyLysGluLysAsnTyrThrSerIleLysGluLysGluProGluGluThrLeuThr
 CGGCAAAGAGAAAAACTATACCTCCATCAAGGAAAAGGAGCCGGAGGAGACGCTGACT

 AspLysAsnGluGluGluGluGluGluLeuLeuLysProValTrpIleArgCysThrH
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 AspLysAsnGluGluGluGluGluGluLeuLeuLysProValTrpIleArgCysThrH
 GACAAAAATGAGGAGGAGGAAGAAGAACTACTGAAGCCCGTGTGGATCCGATGTACCC

 isSerGluAsnTyrTyrSerSerAspProMetAspGlnVal  >>>> Target Int
 |||||||||||||||||||||||||||||||||||||||||           3626 b
 isSerGluAsnTyrTyrSerSerAspProMetAspGlnVal++               
 ATTCAGAAAACTACTATTCCAGTGACCCCATGGATCAGGTGgt               

 ron 4 >>>>  GlyAspSerThrValValGlyThrSerArgLeuArgAspLeuTyrA
 p           ||||||||||||||||||||||||||||||||||||||||||||||
           ++GlyAspSerThrValValGlyThrSerArgLeuArgAspLeuTyrA
           agGGAGATTCTACTGTAGTTGGAACAAGTAGACTTCGTGACTTGTATG

 spLysPheGluGluGluLeuGlySerArgGlnGluLysAlaLysAlaAlaArgProPr
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 spLysPheGluGluGluLeuGlySerArgGlnGluLysAlaLysAlaAlaArgProPr
 ACAAATTTGAAGAAGAGTTAGGGAGCAGGCAAGAGAAGGCTAAGGCCGCTAGACCTCC

 oTrpGluProProLysThrLysLeuAspGluAspLeu{G}  >>>> Target Intr
 |||||||||||||||||||||||||||||||||||||{|}           3421 bp
 oTrpGluProProLysThrLysLeuAspGluAspLeu{G}++                
 GTGGGAACCTCCGAAAACGAAGCTGGACGAGGATTTA{G}gt                

 on 5 >>>>  {lu}SerSerSerGluSerGluCysGluSerAspGluAspSerThrC
            {||}|||||||||||||||||||||||||||||||||||||||||||
          ++{lu}SerSerSerGluSerGluCysGluSerAspGluAspSerThrC
          ag{AG}AGTTCCAGTGAGTCCGAATGTGAGTCCGATGAAGACAGCACCT

 ysSerSerSerSerAspSerGluValPheAspValIleAlaGluIleLysArgLysLy
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 ysSerSerSerSerAspSerGluValPheAspValIleAlaGluIleLysArgLysLy
 GTTCTAGCAGCTCAGATTCTGAAGTTTTTGACGTTATTGCAGAAATCAAACGCAAAAA

 sAlaHisProAspArgLeuHisAspGluLeuTrpTyrAsnAspProGlyGln  >>>>
 ||||||||||||||||||||||||||||||||||||||||||||||||||||      
 sAlaHisProAspArgLeuHisAspGluLeuTrpTyrAsnAspProGlyGln++    
 GGCTCATCCTGACCGACTTCACGATGAACTTTGGTACAATGACCCAGGCCAGgt    

  Target Intron 6 >>>>  MetAsnAspGlyProLeuCysLysCysSerAlaLy
      3474 bp           |||||||||||||||||||||||||||||||||||
                      ++MetAsnAspGlyProLeuCysLysCysSerAlaLy
                      agATGAATGACGGGCCACTCTGCAAATGCAGCGCAAA

 sAlaArgArgThrGlyIleArgHisSerIleTyrProGlyGluGlu  >>>> Targe
 ||||||||||||||||||||||||||||||||||||||||||||||           6
 sAlaArgArgThrGlyIleArgHisSerIleTyrProGlyGluGlu++          
 AGCGAGACGCACAGGAATTAGGCACAGCATTTATCCCGGAGAAGAGgt          

 t Intron 7 >>>>  AlaIleLysProCysArgProMetThrAsnAsnAlaGlyAr
 773 bp              ||||||||||||||||||||||||||||||||||||||
                ++AsnIleLysProCysArgProMetThrAsnAsnAlaGlyAr
                agAACATCAAGCCTTGTCGTCCGATGACCAACAATGCTGGCAG

 gLeuPheHisTyrArgIleThrValSerProProThrAsnPheLeu  >>>> Targe
 ||||||||||||||||||||||||||||||||||||||||||||||           3
 gLeuPheHisTyrArgIleThrValSerProProThrAsnPheLeu++          
 ACTTTTCCACTATCGGATTACGGTTTCCCCCCCTACAAACTTTTTAgt          

 t Intron 8 >>>>  ThrAspArgProThrValIleGluTyrAspAspHisGluTy
 619 bp           |||||||||||||||||||||||||||||||||||||||||
                ++ThrAspArgProThrValIleGluTyrAspAspHisGluTy
                agACTGACAGGCCAACCGTTATAGAATATGATGATCATGAGTA

 rIlePheGluGlyPheSerMetPheAlaHisAlaProLeuThrAsn  >>>> Targe
 ||||||||||||||||||||||||||||||||||||||||||||||           6
 rIlePheGluGlyPheSerMetPheAlaHisAlaProLeuThrAsn++          
 TATCTTCGAAGGATTTTCTATGTTTGCACATGCCCCCCTGACCAATgt          

 t Intron 9 >>>>  IleProLeuCysLysValIleArgPheAsnIleAspTyrTh
 545 bp           |||||||||||||||||||||||||||||||||||||||||
                ++IleProLeuCysLysValIleArgPheAsnIleAspTyrTh
                agATTCCATTGTGTAAAGTTATTAGATTCAACATAGACTACAC

 rIleHisPheIleGluGluMetMetProGlu  >>>> Target Intron 10 >>>
 |||||||||||||||||||||||||||||||            1529 bp        
 rIleHisPheIleGluGluMetMetProGlu++                         
 AATTCATTTCATTGAAGAGATGATGCCTGAGgt                         

 >  AsnPheCysValLysGlyLeuGluLeuPheSerLeuPheLeuPheArgAspIleL
    |||||||||||||||||||||||||||||||||||||||||||||||||||||||
  ++AsnPheCysValLysGlyLeuGluLeuPheSerLeuPheLeuPheArgAspIleL
  agAATTTTTGTGTAAAAGGGCTTGAACTCTTTTCATTGTTCCTATTCAGAGATATTT

 euGluLeuTyrAspTrpAsnLeuLys{G}  >>>> Target Intron 11 >>>> 
 ||||||||||||||||||||||||||{|}            1346 bp          
 euGluLeuTyrAspTrpAsnLeuLys{G}++                          +
 TGGAATTATACGACTGGAATCTTAAA{G}gt                          a

  {ly}ProLeuPheGluAspSerProProCysCysProArgPheHisPheMetProAr
  {||}|||||||||||||||||||||||||||||||||||||||||||||||||||||
 +{ly}ProLeuPheGluAspSerProProCysCysProArgPheHisPheMetProAr
 g{GT}CCTTTGTTTGAAGACAGTCCTCCTTGCTGCCCAAGATTTCATTTCATGCCACG

 gPheValArgPheLeuPro{A}  >>>> Target Intron 12 >>>>  {sp}Gl
 |||||||||||||||||||{|}           15924 bp           {||}||
 gPheValArgPheLeuPro{A}++                          ++{sp}Gl
 TTTTGTAAGATTTCTTCCA{G}gt                          ag{AT}GG

 yGlyLysGluValLeuSerMetHisGlnIleLeuLeuTyrLeuLeuArgCysSerLys
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 yGlyLysGluValLeuSerMetHisGlnIleLeuLeuTyrLeuLeuArgCysSerLys
 AGGAAAGGAAGTTCTGTCCATGCACCAGATCCTCCTCTACTTACTCCGGTGTAGCAAA

 AlaLeuValProGluGluGluIleAlaAsnMetLeuGlnTrpGluGluLeuGluTrpG
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 AlaLeuValProGluGluGluIleAlaAsnMetLeuGlnTrpGluGluLeuGluTrpG
 GCACTGGTCCCTGAGGAGGAGATTGCCAATATGCTGCAGTGGGAAGAACTTGAGTGGC

 lnLysTyrAlaGluGluCysLysGlyMetIleValThrAsnProGlyThr  >>>> T
 ||||||||||||||||||||||||||||||||||||||||||||||||||        
 lnLysTyrAlaGluGluCysLysGlyMetIleValThrAsnProGlyThr++      
 AGAAATATGCCGAGGAATGCAAAGGCATGATCGTGACCAACCCTGGGACGgt      

 arget Intron 13 >>>>  LysProSerSerValArgIleAspGlnLeuAspArg
     4592 bp           ||||||||||||||||||||||||||||||||||||
                     ++LysProSerSerValArgIleAspGlnLeuAspArg
                     agAAACCAAGTTCTGTCCGCATTGATCAACTGGATCGT

 GluGlnPheAsnProAspValIleThrPheProIleIleValHisPheGlyIleArgP
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 GluGlnPheAsnProAspValIleThrPheProIleIleValHisPheGlyIleArgP
 GAACAGTTCAACCCTGATGTGATTACTTTCCCAATTATTGTCCACTTTGGGATACGCC

 roAlaGlnLeuSerTyrAlaGlyAspPro{Gl}  >>>> Target Intron 14 >
 |||||||||||||||||||||||||||||{||}            1701 bp      
 roAlaGlnLeuSerTyrAlaGlyAspPro{Gl}++                       
 CTGCACAGTTGAGTTATGCAGGAGACCCA{CA}gt                       

 >>>  {n}TyrGlnLysLeuTrpLysSerTyrValLysLeuArgHisLeuLeuAlaAs
      {|}||||||||||||||||||||||||||||||||||||||||||||||||||
    ++{n}TyrGlnLysLeuTrpLysSerTyrValLysLeuArgHisLeuLeuAlaAs
    ag{G}TATCAGAAACTGTGGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCAAA

 nSerProLysValLysGlnThrAspLysGlnLysLeuAlaGlnArgGlu  >>>> Ta
 |||||||||||||||||||||||||||||||||||||||||||||||||         
 nSerProLysValLysGlnThrAspLysGlnLysLeuAlaGlnArgGlu++       
 TAGTCCCAAAGTCAAACAAACTGACAAGCAAAAGCTGGCACAGAGGGAGgt       

 rget Intron 15 >>>>  GluAlaLeuGlnLysIleArgGlnLysAsnThrMetA
    1372 bp           |||||||||||||||||||||||||||||||||||||
                    ++GluAlaLeuGlnLysIleArgGlnLysAsnThrMetA
                    agGAAGCACTCCAAAAAATACGACAGAAGAATACAATGA

 rgArgGluValThrValGluLeuSerSerGlnGlyPheTrpLysThrGlyIleArgSe
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 rgArgGluValThrValGluLeuSerSerGlnGlyPheTrpLysThrGlyIleArgSe
 GGCGGGAGGTAACCGTGGAGCTAAGTAGCCAGGGATTCTGGAAAACCGGCATCCGTTC

 rAspValCysGln  >>>> Target Intron 16 >>>>  HisAlaMetMetLeu
 |||||||||||||            8804 bp           |||||||||||||||
 rAspValCysGln++                          ++HisAlaMetMetLeu
 TGATGTCTGCCAGgt                          agCATGCCATGATGCTG

 ProValLeuThrHisHisIleArgTyrHisGlnCysLeuMetHisLeuAspLysLeuI
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 ProValLeuThrHisHisIleArgTyrHisGlnCysLeuMetHisLeuAspLysLeuI
 CCTGTTTTGACCCATCATATCCGCTACCACCAATGCCTAATGCATCTGGACAAGCTGA

 leGlyTyrThrPheGlnAspArgCysLeuLeuGln  >>>> Target Intron 17
 |||||||||||||||||||||||||||||||||||            517 bp     
 leGlyTyrThrPheGlnAspArgCysLeuLeuGln++                     
 TAGGATATACTTTCCAAGATCGTTGTCTGTTACAGgt                     

  >>>>  LeuAlaMetThrHisProSerHisHisLeuAsnPheGlyMetAsnProAsp
        |||||||||||||||||||||||||||||||||||||||||||||||||||
      ++LeuAlaMetThrHisProSerHisHisLeuAsnPheGlyMetAsnProAsp
      agTTGGCCATGACTCATCCAAGCCATCATTTAAATTTTGGAATGAATCCTGAT

 HisAlaArgAsnSerLeuSerAsnCysGlyIleArgGlnProLysTyrGlyAspArgL
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 HisAlaArgAsnSerLeuSerAsnCysGlyIleArgGlnProLysTyrGlyAspArgL
 CACGCCAGGAATTCTTTATCTAACTGTGGAATTCGGCAGCCCAAATACGGAGACAGAA

 ysValHisHisMetHisMetArgLysLys{G}  >>>> Target Intron 18 >>
 |||||||||||||||||||||||||||||{|}            1416 bp       
 ysValHisHisMetHisMetArgLysLys{G}++                        
 AAGTTCATCACATGCACATGCGTAAGAAA{G}gt                        

 >>  {ly}IleAsnThrLeuIleAsnIleMetSerArgLeuGlyGlnAspAspProTh
     {||}||||||||||||||||||||||||||||||||||||||||||||||||||
   ++{ly}IleAsnThrLeuIleAsnIleMetSerArgLeuGlyGlnAspAspProTh
   ag{GA}ATTAACACCTTAATAAATATTATGTCACGCCTTGGACAAGATGACCCAAC

 rProSer{Ar}  >>>> Target Intron 19 >>>>  {g}IleAsnHisAsnGl
 |||||||{||}           13969 bp           {|}||||||||||||||
 rProSer{Ar}++                          ++{g}IleAsnHisAsnGl
 TCCTTCA{AG}gt                          ag{G}ATTAATCACAATGA

 uArgLeuGluPheLeuGlyAspAlaValValGluPheLeuThr{Se}  >>>> Targ
 |||||||||||||||||||||||||||||||||||||||||||{||}           
 uArgLeuGluPheLeuGlyAspAlaValValGluPheLeuThr{Se}++         
 AAGACTGGAGTTCCTGGGTGATGCTGTGGTTGAATTTCTGACC{AG}gt         

 et Intron 20 >>>>  {r}ValHisLeuTyrTyrLeuPheProSerLeuGluGlu
  6555 bp           {|}||||||||||||||||||||||||||||||||||||
                  ++{r}ValHisLeuTyrTyrLeuPheProSerLeuGluGlu
                  ag{T}GTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAA

 GlyGlyLeuAlaThrTyrArgThrAlaIleValGlnAsnGlnHisLeuAlaMetLeuA
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 GlyGlyLeuAlaThrTyrArgThrAlaIleValGlnAsnGlnHisLeuAlaMetLeuA
 GGAGGATTAGCAACCTACCGGACTGCCATTGTTCAGAATCAGCACCTTGCCATGCTAG

 laLys  >>>> Target Intron 21 >>>>  LysLeuGluLeuAspArgPheMe
 |||||            6832 bp           |||||||||||||||||||||||
 laLys++                          ++LysLeuGluLeuAspArgPheMe
 CAAAGgt                          agAAACTTGAACTGGATCGATTTAT

 tLeuTyrAlaHisGlyProAspLeuCysArgGluSerAspLeuArgHisAlaMetAla
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 tLeuTyrAlaHisGlyProAspLeuCysArgGluSerAspLeuArgHisAlaMetAla
 GCTATACGCTCATGGGCCTGACCTTTGTAGAGAATCGGACCTTCGACATGCAATGGCT

 AsnCysPheGluAlaLeuIle{G}  >>>> Target Intron 22 >>>>  {ly}
 |||||||||||||||||||||{|}            3160 bp           {||}
 AsnCysPheGluAlaLeuIle{G}++                          ++{ly}
 AATTGTTTTGAAGCATTAATA{G}gt                          ag{GA}

 AlaValTyrLeuGluGlySerLeuGluGluAlaLysGlnLeuPheGlyArgLeuLeuP
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 AlaValTyrLeuGluGlySerLeuGluGluAlaLysGlnLeuPheGlyArgLeuLeuP
 GCTGTTTACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGCTATTTGGACGCTTACTCT

 heAsnAspPro  >>>> Target Intron 23 >>>>  AspLeuArgGluValTr
 |||||||||||            5816 bp           |||||||||||||||||
 heAsnAspPro++                          ++AspLeuArgGluValTr
 TTAATGATCCGgt                          agGACCTGCGCGAAGTCTG

 pLeuAsnTyrProLeuHisProLeuGln  >>>> Target Intron 24 >>>>  
 ||||||||||||||||||||||||||||            1564 bp           
 pLeuAsnTyrProLeuHisProLeuGln++                          ++
 GCTCAATTATCCTCTCCACCCACTCCAAgt                          ag

 LeuGlnGluProAsnThrAspArgGlnLeuIleGluThrSerProValLeuGlnLysL
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 LeuGlnGluProAsnThrAspArgGlnLeuIleGluThrSerProValLeuGlnLysL
 CTACAAGAACCAAATACTGATCGACAACTTATTGAGACTTCTCCAGTTCTGCAGAAAC

 euThrGluPheGluGluAlaIleGlyValIlePheThrHisValArgLeuLeuAlaAr
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 euThrGluPheGluGluAlaIleGlyValIlePheThrHisValArgLeuLeuAlaAr
 TTACTGAGTTCGAAGAAGCAATTGGAGTGATTTTTACTCATGTTCGACTTCTGGCAAG

 gAlaPheThrLeuArgThrValGlyPheAsnHisLeuThr{Le}  >>>> Target 
 ||||||||||||||||||||||||||||||||||||||||{||}            10
 gAlaPheThrLeuArgThrValGlyPheAsnHisLeuThr{Le}++            
 GGCATTCACTTTGAGGACTGTGGGATTTAACCATCTGACC{TT}gt            

 Intron 25 >>>>  {u}GlyHisAsnGlnArgMetGluPheLeuGlyAspSerIle
 35 bp           {|}|||||||||||||||||||||||||||||||||||||||
               ++{u}GlyHisAsnGlnArgMetGluPheLeuGlyAspSerIle
               ag{A}GGCCACAACCAGAGGATGGAATTCCTAGGTGACTCCATA

 MetGlnLeuValAlaThrGluTyrLeuPheIleHisPheProAspHisHisGluGlyH
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 MetGlnLeuValAlaThrGluTyrLeuPheIleHisPheProAspHisHisGluGlyH
 ATGCAGCTGGTAGCCACAGAGTACTTATTTATTCATTTCCCAGATCATCATGAAGGAC

 isLeuThr  >>>> Target Intron 26 >>>>  LeuLeuArgSerSerLeuVa
 ||||||||           14001 bp           ||||||||||||||||||||
 isLeuThr++                          ++LeuLeuArgSerSerLeuVa
 ACTTAACTgt                          agTTGTTGCGAAGCTCTTTGGT

 lAsnAsnArgThrGlnAlaLysValAlaGluGluLeuGlyMetGlnGluTyrAlaIle
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 lAsnAsnArgThrGlnAlaLysValAlaGluGluLeuGlyMetGlnGluTyrAlaIle
 GAATAACAGGACTCAAGCCAAGGTAGCAGAGGAGCTGGGCATGCAGGAATATGCCATA

 ThrAsnAspLysThrLysArgProValAlaLeuArgThrLysThrLeuAlaAspLeuL
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 ThrAsnAspLysThrLysArgProValAlaLeuArgThrLysThrLeuAlaAspLeuL
 ACGAACGACAAGACCAAGAGACCCGTGGCCCTTCGGACCAAGACCTTGGCGGACCTCT

 euGlu{S}  >>>> Target Intron 27 >>>>  {er}PheIleAlaAlaLeuT
 |||||{|}            1428 bp           {||}||||||||||||||||
 euGlu{S}++                          ++{er}PheIleAlaAlaLeuT
 TGGAA{T}gt                          ag{CG}TTTATTGCAGCATTGT

 yrIleAspLysAspLeuGluTyrValHisThrPheMetAsnValCysPhePheProAr
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 yrIleAspLysAspLeuGluTyrValHisThrPheMetAsnValCysPhePheProAr
 ACATTGATAAGGATTTGGAATATGTTCATACTTTCATGAATGTCTGCTTCTTTCCACG

 gLeuLys  >>>> Target Intron 28 >>>>  GluPheIleLeuAsnGlnAsp
 |||||||             89 bp            |||||||||||||||||||||
 gLeuLys++                          ++GluPheIleLeuAsnGlnAsp
 ATTAAAAgt                          agGAGTTCATTTTGAATCAGGAT

 TrpAsnAspProLysSerGlnLeuGlnGlnCysCysLeuThrLeuArgThrGluGlyL
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 TrpAsnAspProLysSerGlnLeuGlnGlnCysCysLeuThrLeuArgThrGluGlyL
 TGGAATGACCCCAAATCCCAGCTGCAGCAGTGTTGCCTGACACTGAGGACAGAAGGCA

 ysGluProAspIleProLeuTyr{Ly}  >>>> Target Intron 29 >>>>  {
 |||||||||||||||||||||||{||}            2486 bp           {
 ysGluProAspIleProLeuTyr{Ly}++                          ++{
 AAGAGCCAGATATTCCTCTGTAC{AA}gt                          ag{

 s}ThrLeuGlnThrValGlyProSerHisAlaArgThrTyrThrValAlaValTyrPh
 |}||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 s}ThrLeuGlnThrValGlyProSerHisAlaArgThrTyrThrValAlaValTyrPh
 G}ACTCTGCAGACCGTGGGCCCGTCCCATGCTAGAACTTACACTGTGGCTGTGTATTT

 eLysGlyGluArgIleGlyCysGlyLysGlyPro{Se}  >>>> Target Intron
 ||||||||||||||||||||||||||||||||||{||}            575 bp  
 eLysGlyGluArgIleGlyCysGlyLysGlyPro{Se}++                  
 CAAGGGAGAAAGAATAGGCTGTGGGAAAGGACCA{AG}gt                  

  30 >>>>  {r}IleGlnGlnAlaGluMetGlyAlaAlaMetAspAlaLeuGluLys
           {|}|||||||||||||||||||||||||||||||||||||||||||||
         ++{r}IleGlnGlnAlaGluMetGlyAlaAlaMetAspAlaLeuGluLys
         ag{C}ATTCAGCAAGCAGAAATGGGAGCTGCAATGGATGCACTTGAAAAA

 {T}  >>>> Target Intron 31 >>>>  {yr}AsnPheProGlnMetAlaHis
 {|}            5109 bp           {||}|||||||||||||||||||||
 {T}++                          ++{yr}AsnPheProGlnMetAlaHis
 {T}gt                          ag{AT}AACTTTCCCCAGATGGCCCAT

 GlnLysArgPheIleGluArgLysTyrArgGlnGluLeuLysGluMetArgTrpGluA
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 GlnLysArgPheIleGluArgLysTyrArgGlnGluLeuLysGluMetArgTrpGluA
 CAGAAGCGGTTCATTGAACGGAAATACAGACAGGAGTTGAAGGAGATGAGGTGGGAAA

 rgGluHisGlnGluArgGluProAspGluThrGluAspIleLysLys
 ||||||||||||||||||||||||||||||||||||||   ||||||
 rgGluHisGlnGluArgGluProAspGluThrGluAspMetLysLys
 GAGAGCATCAAGAGAGAGAGCCTGATGAGACCGAGGACATGAAGAAG


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