Basic information |
Official Symbol | ARMCX4 |
Description | armadillo repeat containing, X-linked 4 |
Other Aliases | LL0XNC01-209G1.1, CXorf35 |
Other Description | armadillo repeat containing, X-linked 4 pseudogene; armadillo repeat-containing X-linked protein 4 |
Mutation type | Insert and frame-shift 101-102:R-G^AG |
Location | Scaffold148073_2:81696 90003 + |
Wiki | Wikipedia GeneCard |
Sequence |
ARMCX4 |
ATGTCTGCTGCAGGGTTTGAGATCACTGGAAGCAAAGAAACAAGGATACA TCTGTCTGTATTGCCTGTCTCTAGGTGCAAAGATCTCATGAGTTTATATA TCTATCTTAGGGTGTATCGCCAAGCACGAGAAATAAAGAGGAGGGAATTG CCTCGAACCATTGCAGGTCCACCTCCAGTGGCAGCTGTAGTGGCCTTTGA GTGGCTGAAGACCAGCGCCCTCACAGGCCTACACCCAGACCTACCACCCT TTTTTCTTAGCCCGAGTGAGCCTCCCCAGTACCTTGTTGGTGACTTCAGC GGTGATTACATGGGCCGCATTCAGGAAGTGGGCTGGGCGACTGCAGGACT GATGATCTGGGCTGGCACCTGCTACTACATTTACAAATTAACCAAGGGAA GAGCCCAAAGTGTGAGGAGACTTTCCAGACATGAGTCCAGTGTCGCAATG GAGACTGTGGTTGGGGTACAGAACCAGGCCTTGGCCATGAGTGAAGCCAT GGCTGGGGCTAAGATTGAGACTAGACCCAAGGCCAAGACTGGAGCAGAAA CTGGAGCAGGATGTGGGCCTGGGGCTGAAGTAGAAACCAAAGCCATTGCT ATAGCCAGACCCAAAACCAACACTCAGGCCAACACAATAGCTGAGGTAGA GGTAGAGACCCAATCTGAGGCCAAAGCAGTGTCTGGAACACTGGTCATGA CAGAAGCAGTGTCCAGTAGCCAGTGGACTGAAGCCAAGGCCGAAGCCAAG GTCAAAGCCAGGGAAGTGGCTGTGAAAGAGGCAGTGACCCAAACTGATGC TGAGGCTGGGAAAATAGTCAAAAAGGAGGCAGTGACTCAGACCAAGGCTA AAACTTGGGCACTGATTGCCAGGGCAGAGACCAAGAAGGAAGCAATGACC CAGACCAAAGCTGAAGTTCATGTATCGACTGAAAAAGAGGCAAAGATTAA CAAAGTAGTGGTGGCACAGAGTGAGGCGCTGGCAGTGACCAGAGAAGTGG TCAAGATGGGGACCATGATCAAGACTGAAACTGTGGCTGAGGACAAGGCA AGAACCCTGGAAGAGACTGTGAGTGTGGCTAAGACTCAGTCTGAGGCCAG GCCTGGTGTCACAATTGATGCTAGGGGAAATTCCAATGGCATGTCCAGGG CAGTGGCTGGAGTGGACATGACGTCCTGTGCACAGTCTCAGGCTGTGGCC AAGAGCCAGGGTGATAAAATGCCTGGTACTAGGGTTGAGGACAAGGGGAA TCGCAAAACCATGTCTAAGGCAAGGTCTGGAGCAAATGTGAGGGCTTCTG CTCAGTCTCAACTTGCTGCCAAGGCCCATGCTGAGGCCATGCTTGGGGCA AAGATTGATGCTGGGGGTAATACCAATGCCATGTGTAAGACAGGGGCAGA TATGAGAACTGGTATACAATCTCAGACTGAGGCCAAGAAACAGGCTGAGG TAATATCTGGTGCCAGAGTTGATGGCAAGGGAAATATCAATGCCATATCC AAGGCAATGACTGAAGCTGATGTGAGAGCTGCTGCTCAGCCTCAAGCTGT AGTCAGTGCTCAGGCTGAGGTCATGCCTGATGCCAAGGTTAAAGGCAGAG GCAATCCCAATGCTGTGGCTAAAGCAGGGGCCAGGACAAACTCGAGAGCT GGTTCCCAAGCTGAAGCCTTGCCTGATGTCAGGGATAAGACCAAAGACAA TCCCAGTGTCACGGCCAAGACAGGGCCTGGGACAGATACATTGTCTTATA CACAGCCTCAGCCTGTGTCCAATGTCCAGGCTGATGCCTTGCCTGGTGGC AGAATTAAAGCTAAGGGAAATGCCAGCATCATGTCTAAGGAAGGAACTGG GACAGACACAAAAGCACAGTCTCCTGTTGTGACCCAGAGCCAGGGTGAAG CCTTACCCAGTACTAGAAGTAAGGGTAGGGGCAAAACCAAAGCCAAGTCT AAGGCAGGGATTGGGATAGATATGAAAACCTGTGCACGACCTCAGGCTGG GACCAAGACACAATCTGAGGCCTTGCCTGATTCCAGGGTTGATGGTAAGG GCTATCTTAATGCTATTTCTAGAGCTGATGCTAAGGCAGACCAGAAAGCC TGTGGTCAGCCCCAGACTGTGGCGAATTCCCAGGGTGAGGGCTCAGCTGG TGCCAAGAAGAAAGTCAAGGGTAATCCCAATGCTGTGTCTAAGACAGGGG TTGGGGAAAATACAACAGGCTCTGTCCAGCCTCAGATTGTGGCCAATCCC CAGGGTGAGGCCTTGCCCGGTGCCAAAAAGAAAGTCAAGGGTAATCCCAA TGCTGTGTCTAAGACAGGGGTTGGGGAAAATACAACAGGCTCTGCCCAGC CTCAGGTTGTGGCCAGTCCCCAGGGTGAGGCCTTGCCTGGTACCAAGAAT AAAGTCAAGGGTAATCCTAATGCTGTGTCTAAGGCAGAGGCTGGGGCAGG TACTGTAAGCTCTGCCCAGCCCCAGGTTGTGGCCAATTCTCAGGGTGAGG CCTTGCCCGGTGCCAAGAAAAAAGTCAAGGGTAATCCTAATTCTGTGTCT AAGGCAGAGGCTGGGGCAGGTACTGTAAGCTCTGCCCAGCCCCAGGTTGT GGCCAATTCTCAGGGTGAGGCCTTGCCCGGTGCCAAGAAAAAAGTCAAGG GTAATCCTAATGCTGTGCCTAAGGCAGGGGCTGGCACTGATATAGTGGGT CCTACCCAGTCCCAGGATATGGTTAGTTCCCAGGGTGAGGTATTGCCTAG TGCCAAAAACAAGGTCAGGGGTAATCGCAATGCTGTGCCTAAGGCAGGGG ACAGGACAGATACAGTGGGCTCTGCCCAGCCCCTAGATATATCCAGGTCC CAGGATGAGGTCTTGCCTGGTGCCAAGAATAAGGTCAAGGGAAGTCCCAG TGCTGTGCCTAAAGCAGAAGCTGGGTCAAGTACAGTGGGCTCTGCCCAGT CTCAGCCTACGGCCAGTTTCCAGGGTAAGGCCTTGCCTGGTGCCAAGAAT AAGATCAGGAGCAATTCCAATGCCATGTCTAAAGCAGGGGTTGGGACAGA TACAGTGGGCTCTGCTCAGCCCCAGACTGTGACCAGTTCCCAGAATGAGG CCTTGTTTGGTACCAAGAATAAGGTCAAGAGCAATACCAATACTGTGTCT AAGGCAGAGGCTGGGGCAGGTACAATGGGCTCTGCCAAGTCCCAGACTGT GACCACTTCCCAAAGTGAAACCTTGCTTGGTGCCCGGAATAAGGTCAAGG GTAATTCTAATATTGTGTCTAAAGCAGAGGCTGGGGCAGGTACATTGAGC TCTATCCAGAGCCAGGCTGTGGATAGTTTCCTGGGGGAGAACTTGCTTGG TGCCAGGAATAAGGTCAGAGGCAATGCTAATGCTGCTCCTAAAACAGGGG CTGGGGCAAATAGAAGGGGCTCTACCCAGCCCCAAACTGTGGGAAGTTCC CAGGGTGAAGTCTTGCCTAGGGCAAGGGACAAAGCTATGCCTAGTTCTGA GGTGGAAGCCACAACAGAAGGTGAGGTCTATCCAAAGCGAGAAGCTCTGG CAGTGTCCACTTCTGAGAGTGAGGGTGTGACAGGTGCTGAGGCCTCTAGA AAGACTCAGACTGATATCCATGACTACTACTGGAATGGAATTGGTGTTGA AGATTGGATTGCTGCTGAGCGGTGGATCAAATTTAGGTTTCAGGCCATGG ATGGAGATTGGGAGAATAATATGTCTTGGGCTGATGATGAGAATGAAATT AATGTTGGGGCCTGGAGTGGGTCTTCTGACAAGGCTGGTATTATTAGGTC CTGGACTATGGCTTTTTCTGGGGCCAGCATTAAGTCCTGGACTGGGAATG AGGTTGCTCTTGGGTCCTGGGCTGGACCTGGGGACCAGGCCTGTGGAGGA CTCTGGGCTGGGGGTCAGGCTAGTGAGGGGTCCTGGGCTGGGGACAAGGC CAGTGGGGGTTCTTGGACTGGGATTGAGAACCAGGACAGTGGTGGGTCTT GGGCTTCTGGAAACCAGGCCATTGGAGGGCCCTGGGCTGGGGGTCAGGCC AGTGAAGGGTCTTGGCCTGGGGGCCAGACCAGTGGGATCTCCTGGGCTGG GGAAGAGGCTATTGGAGGGTCTTGGACTGGGAACCAGGACAGTGGAGGAT CTTGGGCTGGGACTGGGGCTGGGAATATGGGTACTATTGGGTACTGGACT GGGGGTGTGGATCAGGCTAGTGGTGGGTCCTGGGCTGGGACTGATCATCA ATCTGGGGGTGGGTCCAAGCCTGTATTTATGGATCAGGCTAATGAAAAAG AATCTTGGGCTGGGACTGGTGGCCAGGCCAGTGAAGGGTCCAGGTTGGGT CCTGAGAACCAGTCTAGTGGAAGGTCTTGGGCTGGGACTGTGGACCGAGT CAGTGGAGGGTCTAGGCTGGGGCTTGTAGACCAGTCCAGTGGAAGTCCCT GGGCTGACACTGAGGACCAGGCTGGTGGAGGGTCTAAGCCAGGGTCCATG GACCAACCAGGTGGAGAGCCCTGGGCTGGGACAGGTAACCAGTCTAGTGG TGGATCTAAACCTAGATTTGAGAATCAGGCCAGTGAAGAAGGGTCCTGGA CTGGAGCTGTTTGCCAGAGTGGTGGTGGGTCCAAGTCTGGACCCGAGGAC CAGTCCAGTGGAACATCCTGGGGTGACTCCAGGAACCAAATCAGTGGAGG ATTCTTGATTGGGGCTGTGAATCAGGCCAGTGGAGATTCCTGGGTTGCTA CTGGGGACCAGGCCAGTGGATGGTTTTGTGTTTACACTGGGAGTCAGGCT AGTGGAGGAGGTTCTTGGGTTGGTGCTGGTGGCCAGGATGTTGGAGAGTC TAGGCTGGAGTTCACAAACCAGTCCGGTAGTGGGTCTTGGGCAGGCACTG GGAATCAGGTCAGTGGAGGGTCCTGGACTGGGACTGGGGATCAAGCTGGT GGGTGTTCCAAACCTGGATTTGAGGATCAGACCATTGGAGGAGGGTTCTG GTCTAGTGCTGGGGATCAGGCCAGTGGAGGGTCTAGTCTGGGGCCTGAAG ATCAGCCCAGTGGAGGAGGTTCCTGGGATGGGATTTGTGGCCAGGTCATT GGAGAATCAAGGCCAGAGCCTGCAGACAAGTCCAGTGGTGGGACCTGGGC TGGCACTGCCAGTCAGGCCAGTGGAAGGTCCTGGGTTGGGCCTGGGGATC AGGTTGATGGCTGTCCCAAATCTGGATTTGAAGACCAGGCCTGTGGAGGA AGCTCCTGGGCTGGTGTTGGGAACCAAGCCAGTGGAGAATCTTGGACTGG CTCTAGGCCTGTGAATGAAGTCAGTGGAGAATCTGGATTGGAGCCTGAGG ATCAAGCCAGTGGAGGGTCCTGGGCTAGGTCTGAGGACCAGACCAGTGGA AGGTTTCAGGTCACTGCTGAGACAGAGGCCAATGAAGGATTCTGGTTTGG GCCTGGGAGTGAAGCCTGTATAGGGACTTGGTGCTGGACAGGGGAAAAGG CTGTTATTATGCCCAGTTCTCATGCCAAGGATGAGGCCAGTACTGAATCC AGATCAGGGGCTGAAGAGGAGGTCATCACAAATTCTAGTGTTGGGGCTGA GAATAAGACTAGTGTTGGGTCCTGGATTGGATCTGAGGAGGCAGCTTACA TGGGTTCCTGTCGGGGGGCTGAGGGTGGGGCTGAGGCTGGGTCTGAAGCG AGAATGGGGTCTTGGTCCTGGAACGGAGATACAACCACTATAGAGTCTAG GCTTGGGGCTGGGGAAGAGGCTGGCATAGAGTCCTGGACTTTGGCTAGGG ATGTAGATGAGGATGAGCTAAGCAGAACGTCCAGCCCTGATATTGAGGAG ATCAGTTTAAGGTCTTTGTTTTGGGCTGAGAGTGAGAATAACAATGAATT CAGATCCAAGAGTGGGAAAGATGTCCATTTTGAGTCTGGGGCTGGAGAAA AGGCCAGCATCCAGGATAAGTTTGAGGCTGCTGGTGGAGTTGATATAAGA TCTTGGTTCTGTGCTGAAAGTGAAAACAGAAGTGAGGACAAATCTGCACC TAAGACTAAAGCCAAAAAGTCAGCTGAGTCAAGAGGCACATACCCATCCA TGGTCCCCGGGGCAGGAATGGGGTCATGGGATGGAGCCATGATCTGGTCA GAAACAAAGTTTCCACACCAAAGTGACGCCAGCTACCCAGTTGAAGATGA GTTCAGAAAGCAGGTCAGGACTGGGGAGAAAATTCGGCCCGGGTCCTGCC GCTGTAAACGTGATGCTAATATGGATCCACGAGAGCTTGAAAAACTCATT TGCATGATTGAGATGACTGAAGATCCTTCTGTTCATGAAATAGCCAATAA TGCTCTATATAACAGTGCTGATTATCCTTATTCCCACGAAGTTGTTCGTA ATGTAGGTGGAATCTCAGTTATTGAAAGCTTACTCAATAATCCCCACCCC AGTGTTAGGCAGAAGGCTTTAAATGCATTGAATAATATCTCAGTTGCTGC TGAAAACCATAGGAAGGTTAAAACATATTTAAATCAAGTCTGTGAAGACA CAGTCACCTATCCCTTGAATTCAAATGTGCAGCTGGCTGGACTAAGATTG ATAAGGCATCTGACTATTACTAGTGAATATCAGCATATGGTTACAAACTA TATCTCAGAATTTCTTCGTTTGTTAACTGTGGGAAGTGGAGAAACTAAAG ACCATGTTTTGGGAATGCTCTTGAATTTCTCAAAAAATCCATCTATGACA AAAGACTTGCTCATTGCTAATGCTCCAACAACACTAATGAATATTTTTAG CAAGAAAGAGACAAAAGAGAATATTCTTAATGCTCTTTCGTTATTTGAAA ATATAAATTACCACTTTAAAAGAAGAGCAAAAGCATTTACTCAGGACAAG TTTAATAAAAATTCCCTTTATTTCCTATTCCAACGACCTAAAGCATGTGC CAAGAAACTTCGAGTCTTAGCAGCAGAATGTAATGACCCTGAGGTGAAAG AAAGAGTTGAGCTATTAATAAGTAAACTC |
Alignment |
Align specie | Pep length | Pep align begin | Pep align end | Mutaion type and postion |
Human | 2394 | 1 | 2394 | Insert and frame-shift 101-102:R-G^AG |
Instructions: *** -> premature termination codon; # -> frame shift Alignment: Human pep sequence ---> |||||||||||||||||||||| Treeshrew pep sequence ---> Treeshrew cds sequence ---> MetSerAlaAlaGlyLeuLysIleThrGlySerLysGluThrLysArg >>>> Targe ||||||||||||||| ||||||||||||||||||||| MetSerAlaAlaGlyPheGluIleThrGlySerLysGluThrArgIle-+ ATGTCTGCTGCAGGGTTTGAGATCACTGGAAGCAAAGAAACAAGGATAtt t Intron 1 >>>> ArgLeuLeuLeuIleSerIleAspTrpSerArgAspLeuMetA 306 bp ||| ||||||||| +-HisLeuSerValLeuProValSerArgCysLysAspLeuMetS acCATCTGTCTGTATTGCCTGTCTCTAGGTGCAAAGATCTCATGA snLeuCysIleTyrPheArgValTyrCysGlnGluLysGlnGluGluArgArgGluLeuP ||| |||||| ||||||||| ||| ||||||||||||| erLeuTyrIleTyrLeuArgValTyrArgGlnAlaArgGluIleLysArgArgGluLeuP GTTTATATATCTATCTTAGGGTGTATCGCCAAGCACGAGAAATAAAGAGGAGGGAATTGC roArgIleIleThr{G} >>>> Target Intron 2 >>>> {ly}ProProProG ||||| ||| {|} 341 bp {||}||||||||| roArgThrIleAla{G}++ ++{ly}ProProProV CTCGAACCATTGCA{G}gt ag{GT}CCACCTCCAG luAlaAlaValValAlaPheGluTrpLeuLysThrSerThrLeuThrGlyLeuHisProG |||||||||||||||||||||||||||||||||||| |||||||||||||||||| alAlaAlaValValAlaPheGluTrpLeuLysThrSerAlaLeuThrGlyLeuHisProA TGGCAGCTGTAGTGGCCTTTGAGTGGCTGAAGACCAGCGCCCTCACAGGCCTACACCCAG lnLeuProLeuSerLeuProGlnProGluCysAlaLeuProTyrLeuValArgAlaPheS |||||| ||| ||||||||| |||| spLeuProProPhePheLeuSerProSerGluProProGlnTyrLeuValGlyAspPheS ACCTACCACCCTTTTTTCTTAGCCCGAGTGAGCCTCCCCAGTACCTTGTTGGTGACTTCA erArg--GlyAspTyrMetGlyArgIleGlnGluValGlyTrpValThrAlaGlyLeuVa || ##|||||||||||||||||||||||||||||||||||| |||||||||||| er---##GlyAspTyrMetGlyArgIleGlnGluValGlyTrpAlaThrAlaGlyLeuMe GC---AGGGTGATTACATGGGCCGCATTCAGGAAGTGGGCTGGGCGACTGCAGGACTGAT lIleTrpAlaGlyThrCysTyrTyrIleTyrLysPheThrLysGlyArgAlaGlnSerVa ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| tIleTrpAlaGlyThrCysTyrTyrIleTyrLysLeuThrLysGlyArgAlaGlnSerVa GATCTGGGCTGGCACCTGCTACTACATTTACAAATTAACCAAGGGAAGAGCCCAAAGTGT lArgThrLeuAlaArgAsnGlySerThrValLysMetGluThrValValGlyValGlnSe |||| ||| ||| ||| ||| |||||||||||||||||||||||| lArgArgLeuSerArgHisGluSerSerValAlaMetGluThrValValGlyValGlnAs GAGGAGACTTTCCAGACATGAGTCCAGTGTCGCAATGGAGACTGTGGTTGGGGTACAGAA rGlnThrLeuAlaIleAsnGlu<-><-><-><->AlaGluIleLysThrLysProGlnVa ||| |||||| ||| ||| ||| ||| ||| nGlnAlaLeuAlaMetSerGluAlaMetAlaGlyAlaLysIleGluThrArgProLysAl CCAGGCCTTGGCCATGAGTGAAGCCATGGCTGGGGCTAAGATTGAGACTAGACCCAAGGC lGluIleGlyAlaGluThrGlyAlaArgSerGlyProArgAlaGluValGluThrLysAl |||||||||||||||||| |||||| |||||||||||||||||||| aLysThrGlyAlaGluThrGlyAlaGlyCysGlyProGlyAlaGluValGluThrLysAl CAAGACTGGAGCAGAAACTGGAGCAGGATGTGGGCCTGGGGCTGAAGTAGAAACCAAAGC aThrAlaIleAlaIleHisArgAlaAsnSerGlnAlaLysAlaMetValGlyAlaGluPr | ||||||||| ||| |||||| ||| aIleAlaIleAlaArgProLysThrAsnThrGlnAlaAsnThrIleAlaGluValGluVa CATTGCTATAGCCAGACCCAAAACCAACACTCAGGCCAACACAATAGCTGAGGTAGAGGT oGluThrGlnSerGluSerLysValValAlaGlyThrLeuValMetThrGluAlaValTh ||||||||||||||| ||| ||| ||||||||||||||||||||||||||| lGluThrGlnSerGluAlaLysAlaValSerGlyThrLeuValMetThrGluAlaValSe AGAGACCCAATCTGAGGCCAAAGCAGTGTCTGGAACACTGGTCATGACAGAAGCAGTGTC rLeuThr<-><-><-><-><-><-><->GluValLysAlaLysAlaArgGluValAlaMe ||| ||| |||||||||||||||||| rSerSerGlnTrpThrGluAlaLysAlaGluAlaLysValLysAlaArgGluValAlaVa CAGTAGCCAGTGGACTGAAGCCAAGGCCGAAGCCAAGGTCAAAGCCAGGGAAGTGGCTGT tLysGluAlaValThrGlnThrAspAlaGluAlaGlyLysIleValLysLysGluAlaVa ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lLysGluAlaValThrGlnThrAspAlaGluAlaGlyLysIleValLysLysGluAlaVa GAAAGAGGCAGTGACCCAAACTGATGCTGAGGCTGGGAAAATAGTCAAAAAGGAGGCAGT lThrGlnThrLysAlaLysAlaTrpAlaLeuValAlaLysThrGluAlaLysArgGluAl ||||||||||||||||||| ||||||||| ||| ||| ||| ||||| lThrGlnThrLysAlaLysThrTrpAlaLeuIleAlaArgAlaGluThrLysLysGluAl GACTCAGACCAAGGCTAAAACTTGGGCACTGATTGCCAGGGCAGAGACCAAGAAGGAAGC aMetThrGlnThrLysAlaGluThrHisIleLeuAlaGluLysGluThrGluIleAsnAr |||||||||||||||||||||| ||| ||||||||| |||||| aMetThrGlnThrLysAlaGluValHisValSerThrGluLysGluAlaLysIleAsnLy AATGACCCAGACCAAAGCTGAAGTTCATGTATCGACTGAAAAAGAGGCAAAGATTAACAA gValMetValThrGlnSerGluThrLeuAlaValProArgGluValAlaLysMetGlyAl ||| ||| ||||||||| ||||||||| ||||||||| ||||||||| sValValValAlaGlnSerGluAlaLeuAlaValThrArgGluValValLysMetGlyTh AGTAGTGGTGGCACAGAGTGAGGCGCTGGCAGTGACCAGAGAAGTGGTCAAGATGGGGAC aThrAsnLysThrGlyIleValAspGluThrLysThrArgAlaLeuGluGluThrValSe |||||| ||| ||| ||| ||| ||||||||||||||||| rMetIleLysThrGluThrValAlaGluAspLysAlaArgThrLeuGluGluThrValSe CATGATCAAGACTGAAACTGTGGCTGAGGACAAGGCAAGAACCCTGGAAGAGACTGTGAG rValAlaLysThrGlnSerGluAlaArgProGlyAlaThrValAspAlaArgGlyAsnPr |||||||||||||||||||||||||||||||||| ||| ||||||||||||||| rValAlaLysThrGlnSerGluAlaArgProGlyValThrIleAspAlaArgGlyAsnSe TGTGGCTAAGACTCAGTCTGAGGCCAGGCCTGGTGTCACAATTGATGCTAGGGGAAATTC oAsnGlyMetSerArgGluValAlaGlyValAspMetLysSerCysAlaGlnSerGlnAl ||||||||||||||| |||||||||||||||||| |||||||||||||||||||| rAsnGlyMetSerArgAlaValAlaGlyValAspMetThrSerCysAlaGlnSerGlnAl CAATGGCATGTCCAGGGCAGTGGCTGGAGTGGACATGACGTCCTGTGCACAGTCTCAGGC aValThrLysIleGlnGlyAspAspMetProGlyThrGlyValGluAspMetGlyAsnCy |||| ||| ||||||||| |||||||||||| ||||||||| |||||| aValAlaLysSerGlnGlyAspLysMetProGlyThrArgValGluAspLysGlyAsnAr TGTGGCCAAGAGCCAGGGTGATAAAATGCCTGGTACTAGGGTTGAGGACAAGGGGAATCG sLysThrMetSerArgAlaGluSerGlyAlaAspThrArgAlaSerAlaGlnProGlnIl |||||||||||| ||| ||||||||| ||||||||||||||| ||| gLysThrMetSerLysAlaArgSerGlyAlaAsnValArgAlaSerAlaGlnSerGlnLe CAAAACCATGTCTAAGGCAAGGTCTGGAGCAAATGTGAGGGCTTCTGCTCAGTCTCAACT ePheAlaLysThrGlnThrGluAlaIleProGlyAlaLysIleAspAlaGlyGlyAsnTh |||||| |||||| ||||||||||||||||||||||||||||| uAlaAlaLysAlaHisAlaGluAlaMetLeuGlyAlaLysIleAspAlaGlyGlyAsnTh TGCTGCCAAGGCCCATGCTGAGGCCATGCTTGGGGCAAAGATTGATGCTGGGGGTAATAC rAsnAlaMetCysLysValGlyAlaGlyAlaAspValArgAlaCysIleGlnProGlnTh |||||||||||||||| ||||||||| ||| |||||| ||||| rAsnAlaMetCysLys------ThrGlyAlaAspMetArgThrGlyIleGlnSerGlnTh CAATGCCATGTGTAAG------ACAGGGGCAGATATGAGAACTGGTATACAATCTCAGAC rValAlaLysLysGlnAlaGluValThrSerGlyAlaArgValAspGlyArgGlyAsnTh | ||||||||||||||||||||| ||||||||||||||||||||| |||||| rGluAlaLysLysGlnAlaGluValIleSerGlyAlaArgValAspGlyLysGlyAsnIl TGAGGCCAAGAAACAGGCTGAGGTAATATCTGGTGCCAGAGTTGATGGCAAGGGAAATAT rAsnValIleSerLysAlaIleThrGlyAlaAspMetArgAlaAlaAlaGlnProGlnAl ||| |||||||||||| ||| |||||| ||||||||||||||||||||||| eAsnAlaIleSerLysAlaMetThrGluAlaAspValArgAlaAlaAlaGlnProGlnAl CAATGCCATATCCAAGGCAATGACTGAAGCTGATGTGAGAGCTGCTGCTCAGCCTCAAGC aValAlaSerThrHisAlaGluAlaMetSerAspAlaLysValLysAsnArgGlyAsnPr |||| ||| |||||| ||| ||||||||||||||| ||||||||||| aValValSerAlaGlnAlaGluValMetProAspAlaLysValLysGlyArgGlyAsnPr TGTAGTCAGTGCTCAGGCTGAGGTCATGCCTGATGCCAAGGTTAAAGGCAGAGGCAATCC oAsnAlaMetThrLysAlaGlyAlaLysAlaAsnLeuArgAlaAsnSerGlnValGluAl ||||||| |||||||||||| ||| |||||| |||||| ||||| oAsnAlaValAlaLysAlaGlyAlaArgThrAsnSerArgAlaGlySerGlnAlaGluAl CAATGCTGTGGCTAAAGCAGGGGCCAGGACAAACTCGAGAGCTGGTTCCCAAGCTGAAGC aLeuProAspAlaArgAspLysSerArgGlyAsnProAsnValMetAlaLysValGlyAs |||||||||| ||||||||| |||||| ||| |||||| ||| aLeuProAspValArgAspLysThrLysAspAsnProSerValThrAlaLysThrGlyPr CTTGCCTGATGTCAGGGATAAGACCAAAGACAATCCCAGTGTCACGGCCAAGACAGGGCC pGlyThrAspMetLeuSerCysThrGlnProGlnLeuValAlaSerValGlnAlaAspTh ||||||||| |||||| |||||||||||| ||| |||||||||||| oGlyThrAspThrLeuSerTyrThrGlnProGlnProValSerAsnValGlnAlaAspAl TGGGACAGATACATTGTCTTATACACAGCCTCAGCCTGTGTCCAATGTCCAGGCTGATGC rLeuSerAspGlyLysIleLysValArgGlyAsnValAsnThrMetProLysGluGlyAl ||| ||| |||||| |||||| ||| ||||||||| aLeuProGlyGlyArgIleLysAlaLysGlyAsnAlaSerIleMetSerLysGluGlyTh CTTGCCTGGTGGCAGAATTAAAGCTAAGGGAAATGCCAGCATCATGTCTAAGGAAGGAAC aGlyValAspMetLysAlaGlnGly<-><->MetAlaGlnSerGlnGlyGluAlaLeuPr ||| ||| ||||||||| ||||||||||||||||||||||| rGlyThrAspThrLysAlaGlnSerProValValThrGlnSerGlnGlyGluAlaLeuPr TGGGACAGACACAAAAGCACAGTCTCCTGTTGTGACCCAGAGCCAGGGTGAAGCCTTACC oAsnThrArgGlyLysAlaArgGlyLysAlaLysAlaLysCysLysThrGlyProGlyMe | |||||| ||| ||||||||| ||||||||| ||| ||| ||| oSerThrArgSerLysGlyArgGlyLysThrLysAlaLysSerLysAlaGlyIleGlyIl CAGTACTAGAAGTAAGGGTAGGGGCAAAACCAAAGCCAAGTCTAAGGCAGGGATTGGGAT tAspMetLysThrCysThrGlnProGlnAlaGlyValLysThrProAlaGluAlaLeuLe ||||||||||||||| |||||||||||| |||||| ||||||||| eAspMetLysThrCysAlaArgProGlnAlaGlyThrLysThrGlnSerGluAlaLeuPr AGATATGAAAACCTGTGCACGACCTCAGGCTGGGACCAAGACACAATCTGAGGCCTTGCC uAspSerArgValAspGlyArgGlyAsnProAsnAlaThrSerLysAlaGlyThrLysAl |||||||||||||||||| ||| |||||| ||| ||| ||||| oAspSerArgValAspGlyLysGlyTyrLeuAsnAlaIleSerArgAlaAspAlaLysAl TGATTCCAGGGTTGATGGTAAGGGCTATCTTAATGCTATTTCTAGAGCTGATGCTAAGGC aAspGlnArgValCysGlyGlnProLeuValValAlaAsnProGlnGlyGluAlaLeuPr ||||||| |||||||||||| ||||||||| ||||||||| aAspGlnLysAlaCysGlyGlnProGlnThrValAlaAsnSerGlnGlyGluGlySerAl AGACCAGAAAGCCTGTGGTCAGCCCCAGACTGTGGCGAATTCCCAGGGTGAGGGCTCAGC oGlyAlaLysAsnLysValLysGlyAsnProHisThrValLeuLysValGlyAlaGlyGl ||||||||| |||||||||||||||||| ||| ||| ||| ||||| aGlyAlaLysLysLysValLysGlyAsnProAsnAlaValSerLysThrGlyValGlyGl TGGTGCCAAGAAGAAAGTCAAGGGTAATCCCAATGCTGTGTCTAAGACAGGGGTTGGGGA uGlyThrThrAspSerAlaGlnProGluAlaValValSerPheGlnGlyGluAlaLeuLe | |||||| ||| |||||| ||| ||||||||||||||| uAsnThrThrGlySerValGlnProGlnIleValAlaAsnProGlnGlyGluAlaLeuPr AAATACAACAGGCTCTGTCCAGCCTCAGATTGTGGCCAATCCCCAGGGTGAGGCCTTGCC uGlyThrLysAsnLysValLysGlyAsnProAsnValValLeuLysAlaGluValGlyGl ||| ||| ||||||||||||||||||||| ||| ||| |||||||| oGlyAlaLysLysLysValLysGlyAsnProAsnAlaValSerLysThrGlyValGlyGl CGGTGCCAAAAAGAAAGTCAAGGGTAATCCCAATGCTGTGTCTAAGACAGGGGTTGGGGA uGlyAlaMetGlyThrAlaGlnLeuGlnIleMetAlaSerSerLysGlyGluAlaLeuLe | ||| |||||| ||| |||||| |||||||||||| uAsnThrThrGlySerAlaGlnProGlnValValAlaSerProGlnGlyGluAlaLeuPr AAATACAACAGGCTCTGCCCAGCCTCAGGTTGTGGCCAGTCCCCAGGGTGAGGCCTTGCC uAspSerLysAsnLysValLysGlyAsnSerAsnAlaValSerLysAlaGlyAlaGlyTh ||||||||||||||||||||| |||||||||||||||||| |||||| oGlyThrLysAsnLysValLysGlyAsnProAsnAlaValSerLysAlaGluAlaGlyAl TGGTACCAAGAATAAAGTCAAGGGTAATCCTAATGCTGTGTCTAAGGCAGAGGCTGGGGC rAspThrThrGlySerValGlnProGlnIleValAlaAsnSerGlnGlyGluValLeuPr ||| ||| ||||||||| ||||||||||||||||||||| ||||| aGlyThrValSerSerAlaGlnProGlnValValAlaAsnSerGlnGlyGluAlaLeuPr AGGTACTGTAAGCTCTGCCCAGCCCCAGGTTGTGGCCAATTCTCAGGGTGAGGCCTTGCC oGlyAlaLysAsnLysIleArgGlyAsnProThrThrValProAsnSerGlyValGlyPr |||||||||| ||| ||||||||| ||| ||| oGlyAlaLysLysLysValLysGlyAsnProAsnSerValSerLysAlaGluAlaGlyAl CGGTGCCAAGAAAAAAGTCAAGGGTAATCCTAATTCTGTGTCTAAGGCAGAGGCTGGGGC oTyrThrThrAspSerAlaArgLeuGlnAlaValAlaAsnSerGlnGlyGluValLeuPr ||| |||||| ||| ||||||||||||||||||||| ||||| aGlyThrValSerSerAlaGlnProGlnValValAlaAsnSerGlnGlyGluAlaLeuPr AGGTACTGTAAGCTCTGCCCAGCCCCAGGTTGTGGCCAATTCTCAGGGTGAGGCCTTGCC oGlyAlaLysAsnLysValLysAlaAsnLeuAsnAlaValSerLysAlaGluAlaGlyMe |||||||||| ||||||||| ||| ||||||||| |||||| |||||| oGlyAlaLysLysLysValLysGlyAsnProAsnAlaValProLysAlaGlyAlaGlyTh CGGTGCCAAGAAAAAAGTCAAGGGTAATCCTAATGCTGTGCCTAAGGCAGGGGCTGGCAC tGlyAlaThrGlySerValGlnProGlnAlaValAlaAsnSerHisCysGluThrLeuPr ||| ||| ||| ||| ||| ||||| rAspIleValGlyProThrGlnSerGlnAspMetValSerSerGlnGlyGluValLeuPr TGATATAGTGGGTCCTACCCAGTCCCAGGATATGGTTAGTTCCCAGGGTGAGGTATTGCC oGlyAlaLysAsnLysValArgGlyAsnTrpAsnAlaValSerLysAlaGlyAlaGlyMe | |||||||||||||||||||||||| ||||||||| ||||||||| oSerAlaLysAsnLysValArgGlyAsnArgAsnAlaValProLysAlaGlyAspArgTh TAGTGCCAAAAACAAGGTCAGGGGTAATCGCAATGCTGTGCCTAAGGCAGGGGACAGGAC tAspThrArgGlySerAlaGlnProGlnAlaValAlaAsnSerGlnGlyGluValLeuPr |||||| ||||||||||||||| |||||| ||||||||||| rAspThrValGlySerAlaGlnProLeuAspIleSerArgSerGlnAspGluValLeuPr AGATACAGTGGGCTCTGCCCAGCCCCTAGATATATCCAGGTCCCAGGATGAGGTCTTGCC oGlyAlaLysAsnLysValLysGlyAsnProAsnValValSerLysAlaGlyAlaArgGl ||||||||||||||||||||||||| ||| ||| |||||| ||| oGlyAlaLysAsnLysValLysGlySerProSerAlaValProLysAlaGluAlaGlySe TGGTGCCAAGAATAAGGTCAAGGGAAGTCCCAGTGCTGTGCCTAAAGCAGAAGCTGGGTC uAspThrValGlySerThrGlnProGlnValLeuAlaSerSerGlnArgGluThrLeuPr |||||||||||| ||| ||| |||||| ||| ||||| rSerThrValGlySerAlaGlnSerGlnProThrAlaSerPheGlnGlyLysAlaLeuPr AAGTACAGTGGGCTCTGCCCAGTCTCAGCCTACGGCCAGTTTCCAGGGTAAGGCCTTGCC oGlyAlaArgAsnLysValLysGlyAsnSerAsnValValSerLysAlaGlyAlaArgGl ||||||| |||||| ||||||||| |||||||||||| oGlyAlaLysAsnLysIleArgSerAsnSerAsnAlaMetSerLysAlaGlyValGlyTh TGGTGCCAAGAATAAGATCAGGAGCAATTCCAATGCCATGTCTAAAGCAGGGGTTGGGAC uAspThrMetGlySerAlaGlnProGlnValValAlaAsnSerGln{A} >>>> Targ |||||| |||||||||||||||||| ||| ||||||{ } rAspThrValGlySerAlaGlnProGlnThrValThrSerSerGln{A}++ AGATACAGTGGGCTCTGCTCAGCCCCAGACTGTGACCAGTTCCCAG{A}gt et Intron 3 >>>> {rg}GluThrLeuProGlyAlaArgAsnLysValLysGlyAs 552 bp { }||| ||| ||| |||||||||||| || -+{sn}GluAlaLeuPheGlyThrLysAsnLysValLysSerAs gg{AT}GAGGCCTTGTTTGGTACCAAGAATAAGGTCAAGAGCAA nSerAsnAlaIleSerLysAlaGluAlaGlyAlaGlyIleMetGlySerValGlnValGl | ||| |||||||||||||||||||||||| ||||||||| || nThrAsnThrValSerLysAlaGluAlaGlyAlaGlyThrMetGlySerAlaLysSerGl TACCAATACTGTGTCTAAGGCAGAGGCTGGGGCAGGTACAATGGGCTCTGCCAAGTCCCA nValValAlaSerPheGlnGlyGluValLeuProGlyAlaLysAsnLysValArgGlyAs | ||| ||| ||| ||| |||||| ||||||||| ||||| nThrValThrThrSerGlnSerGluThrLeuLeuGlyAlaArgAsnLysValLysGlyAs GACTGTGACCACTTCCCAAAGTGAAACCTTGCTTGGTGCCCGGAATAAGGTCAAGGGTAA nSerAsnAlaValProLysAlaGluAlaGlyAlaAspThrValGlySerAlaGlnProGl ||||||| ||| |||||||||||||||||| ||| ||| ||| || nSerAsnIleValSerLysAlaGluAlaGlyAlaGlyThrLeuSerSerIleGlnSerGl TTCTAATATTGTGTCTAAAGCAGAGGCTGGGGCAGGTACATTGAGCTCTATCCAGAGCCA nAlaValAlaAsnSerGlnSerGluThrLeuLeuGlyAlaArgAsnLysValLysGlyAs ||||||| ||| |||||||||||||||||||||||| ||||| nAlaValAspSerPheLeuGlyGluAsnLeuLeuGlyAlaArgAsnLysValArgGlyAs GGCTGTGGATAGTTTCCTGGGGGAGAACTTGCTTGGTGCCAGGAATAAGGTCAGAGGCAA nThrIleAlaValProLysAlaGlyThrGlyAlaGlyThrArgHisSerAlaGlnProGl | ||| |||||| ||| |||||| ||| ||| |||||||| nAlaAsnAlaAlaProLysThrGlyAlaGlyAlaAsnArgArgGlySerThrGlnProGl TGCTAATGCTGCTCCTAAAACAGGGGCTGGGGCAAATAGAAGGGGCTCTACCCAGCCCCA nIleValAlaGlySerGlnGlyGluThrLeuProGlyAlaArgAspLysSerMetSerTh | ||| |||||||||||| |||||| |||||||||||| ||| nThrValGlySerSerGlnGlyGluValLeuProArgAlaArgAspLysAlaMetProSe AACTGTGGGAAGTTCCCAGGGTGAAGTCTTGCCTAGGGCAAGGGACAAAGCTATGCCTAG rSerGluAlaGluAlaThrAlaGluAspGluAlaTyrAlaLysProGluAlaGluAlaMe |||||| ||||||||| ||| ||| ||| ||| |||||| ||| rSerGluValGluAlaThrThrGluGlyGluValTyrProLysArgGluAlaLeuAlaVa TTCTGAGGTGGAAGCCACAACAGAAGGTGAGGTCTATCCAAAGCGAGAAGCTCTGGCAGT tProThrSerGluSerGluGlyGlySerGlyThrGlnAlaCysArgLysThrGlnProAs |||||||||||||||||| ||| ||| |||||||||||| lSerThrSerGluSerGluGlyValThrGlyAlaGluAlaSerArgLysThrGlnThrAs GTCCACTTCTGAGAGTGAGGGTGTGACAGGTGCTGAGGCCTCTAGAAAGACTCAGACTGA nIleHisAspTyrTyrTrpAsnGlyIleGlyValGluAspTrpIleAlaAlaGluArgTr ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| pIleHisAspTyrTyrTrpAsnGlyIleGlyValGluAspTrpIleAlaAlaGluArgTr TATCCATGACTACTACTGGAATGGAATTGGTGTTGAAGATTGGATTGCTGCTGAGCGGTG pIleLysPheArgPheGlnThrMetAspGlyAspTrpGluAsnSerValSerTrpAlaAs ||||||||||||||||||| ||||||||||||||||||||| ||||||||||| pIleLysPheArgPheGlnAlaMetAspGlyAspTrpGluAsnAsnMetSerTrpAlaAs GATCAAATTTAGGTTTCAGGCCATGGATGGAGATTGGGAGAATAATATGTCTTGGGCTGA pAspGluAsnGluAlaSerIleGlySerTrpSerGlyAlaSerAspLysAlaGlyIleIl ||||||||||||| ||| ||||||||| |||||||||||||||||||| pAspGluAsnGluIleAsnValGlyAlaTrpSerGlySerSerAspLysAlaGlyIleIl TGATGAGAATGAAATTAATGTTGGGGCCTGGAGTGGGTCTTCTGACAAGGCTGGTATTAT eArgSerTrpAlaValAlaCysAspGluThrSerValLysSerTrpAlaGlyAlaArgAl |||||||||| ||| ||| ||||||||| ||| eArgSerTrpThrMetAlaPheSerGlyAlaSerIleLysSerTrpThrGly-------- TAGGTCCTGGACTATGGCTTTTTCTGGGGCCAGCATTAAGTCCTGGACTGGG-------- aGluAsnValValGlyIleGlyThrTrpAlaArgAlaGlyGluGlnAlaSerGlyGlyLe ||| ||| ||| |||||| ||| |||||| |||||||| ----AsnGluValAlaLeuGlySerTrpAlaGlyProGlyAspGlnAlaCysGlyGlyLe ----AATGAGGTTGCTCTTGGGTCCTGGGCTGGACCTGGGGACCAGGCCTGTGGAGGACT uTrpAlaGlyGlyGlnThrSerGluGlyThrTrpAlaGlyAspLysAlaSerGlyGlyAl |||||||||||||||| ||||||||| ||||||||||||||||||||||||||| uTrpAlaGlyGlyGlnAlaSerGluGlySerTrpAlaGlyAspLysAlaSerGlyGlySe CTGGGCTGGGGGTCAGGCTAGTGAGGGGTCCTGGGCTGGGGACAAGGCCAGTGGGGGTTC a{Tr} >>>> Target Intron 4 >>>> {p}ThrGlyAlaGluAsnGlnAlaSe {||} 36 bp {|}|||||| ||||||||| || r{Tr}+- -+{p}ThrGlyIleGluAsnGlnAspSe T{TG}gg tg{G}ACTGGGATTGAGAACCAGGACAG rGlyGlySerTrpAlaLeuAlaGlyAsnGlnAlaIleGlyGluLeuTrpAlaAlaGlyGl |||||||||||||||| |||||||||||||||||| |||||| ||||| rGlyGlySerTrpAla---SerGlyAsnGlnAlaIleGlyGlyProTrpAlaGlyGlyGl TGGTGGGTCTTGGGCT---TCTGGAAACCAGGCCATTGGAGGGCCCTGGGCTGGGGGTCA nAlaSerAspGlySerTrpProGlyGlyGlnAlaSerGlyValSerTrpValGlyGluGl ||||||| ||||||||||||||||||||| |||||| |||||| |||||||| nAlaSerGluGlySerTrpProGlyGlyGlnThrSerGlyIleSerTrpAlaGlyGluGl GGCCAGTGAAGGGTCTTGGCCTGGGGGCCAGACCAGTGGGATCTCCTGGGCTGGGGAAGA uAlaIleGlyGlySerTrpThrGlyAlaGluAsnGlnAlaSerGluGlySerTrpAlaGl ||||||||||||||||||||||||| |||||| ||| |||||||||||||| uAlaIleGlyGlySerTrpThrGly------AsnGlnAspSerGlyGlySerTrpAlaGl GGCTATTGGAGGGTCTTGGACTGGG------AACCAGGACAGTGGAGGATCTTGGGCTGG yAlaGlyAlaGlyAsnMetSerSerValSerTyrTrpAlaGlyValValAspGlnAlaGl | ||||||||||||||| |||||| ||| |||||||||||| yThrGlyAlaGlyAsnMetGlyThrIleGlyTyrTrpThrGlyGlyValAspGlnAlaSe GACTGGGGCTGGGAATATGGGTACTATTGGGTACTGGACTGGGGGTGTGGATCAGGCTAG yGlyGlySerTrpAlaGlyThrSerAspGlnSerGlyGlyGlySerLysProArgPheGl ||||||||||||||||||||| |||||||||||||||||||||||| ||| rGlyGlySerTrpAlaGlyThrAspHisGlnSerGlyGlyGlySerLysProValPheMe TGGTGGGTCCTGGGCTGGGACTGATCATCAATCTGGGGGTGGGTCCAAGCCTGTATTTAT uAspGlnAlaSerGlyGluGlySerTrpAlaGlyAlaGlyGlyGlnAlaSerGlyGlySe ||||||||| |||||||||||| ||||||||||||||| ||||| tAspGlnAlaAsnGluLysGluSerTrpAlaGlyThrGlyGlyGlnAlaSerGluGlySe GGATCAGGCTAATGAAAAAGAATCTTGGGCTGGGACTGGTGGCCAGGCCAGTGAAGGGTC rMetLeuGlyProGluAspGlnSerSerGlyArgSerTrpAlaAspThrAlaAspGlnAl | |||||||||||| |||||||||||||||||||||||| ||| ||| rArgLeuGlyProGluAsnGlnSerSerGlyArgSerTrpAlaGlyThrValAspArgVa CAGGTTGGGTCCTGAGAACCAGTCTAGTGGAAGGTCTTGGGCTGGGACTGTGGACCGAGT aSerGlyGlySerArgLeuGlyHisValAspGlnSerSerGlyGlyAlaTrpAlaGlyTh ||||||||||||||||||||| |||||||||||||||||| |||||| || lSerGlyGlySerArgLeuGlyLeuValAspGlnSerSerGlySerProTrpAlaAspTh CAGTGGAGGGTCTAGGCTGGGGCTTGTAGACCAGTCCAGTGGAAGTCCCTGGGCTGACAC rLeuAspGlnSerGlyGlyGlySerLysProArgPheGluAsnGlnThrThrGluGluGl | |||||| |||||||||||||||||| ||| ||| rGluAspGlnAlaGlyGlyGlySerLysProGlySerMetAspGlnProGlyGlyGluPr TGAGGACCAGGCTGGTGGAGGGTCTAAGCCAGGGTCCATGGACCAACCAGGTGGAGAGCC ySerTrpAlaGlyAlaGlyGlyGlnAlaGlyGlyGlySerLysValGlyProGluAspGl |||||| |||||| ||| || oTrpAlaGlyThrGlyAsnGlnSerSerGlyGly---SerLysProArgPheGluAsnGl CTGGGCTGGGACAGGTAACCAGTCTAGTGGTGGA---TCTAAACCTAGATTTGAGAATCA nSerSerGlyArgSerTrpAlaAsnSerGlyAspGlnIleSerGlyGlyPheLeuValGl | ||| nAlaSer----------------------------------------- // |